Knockdown of HMGA1 expression by short/small hairpin RNA inhibits growth of ovarian carcinoma cells

Biotechnology and Applied Biochemistry
Yinglan LiuGuangmei Zhang

Abstract

The aim of the current study was to investigate the influence of downregulating high-mobility group protein A1 (HMGA1) on the tumor gene and the mechanisms underlying the antitumor of HMGA1. The efficient short/small hairpin RNAs (shRNAs) of HMGA1 were constructed and transfected into human ovarian carcinoma OVCAR cells. The changes were identified by reverse transcription PCR (RT-PCR), Western blotting, methyl thiazolyl tetrazolium, and invasion assay. The knockdown of HMGA1 expression in OVCAR cells could obviously change cell morphology, decrease cell proliferation, and reduce invasion in vitro. BALB/C nude mice injected with OVCAR cells transfected HMGA1 shRNA showed a significantly lower tumor weight and volume than those in the control group. Taken together, HMGA1 knockdown could reduce the growth and metastasis potentials of OVCAR cells.

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Citations

Aug 27, 2016·Experimental & Molecular Medicine·Dae Kyoung KimJae Ho Kim
Jan 10, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Cuiling QiBo Wei
Jan 8, 2019·Journal of Cellular and Molecular Medicine·Yuhong WangLingchuan Guo
Feb 20, 2016·Oncotarget·Marco De MartinoFrancesco Esposito
Jun 2, 2018·International Journal of Cell Biology·Vincenzo GiancottiClaudio Rizzi

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