Abstract
The purpose of the article is to investigate the role of IARS2 in proliferation, apoptosis, and cell cycle of gastric cancer (GC) cells in vitro. The IARS2-shRNA lentiviral vector was established and used to infect the GC cell line AGS. qRT-PCR and Western blot were employed to determine the efficiency of IARS2 knockdown. The effects of IARS2 knockdown on cell proliferation, cell clone formation, and cell cycle were assessed by MTT assay, colony formation assay, and flow cytometer analysis, respectively. Finally, a PathScan Antibody Array Kit was used to detect the expression levels of cell cycle-related proteins after IARS2 knockdown in AGS cells to elucidate the underlying mechanisms. Compared with negative control group, IARS2 was significantly knocked down by transfection with lentivirus encoding shRNA of IARS2 in AGS cells. IARS2 knockdown significantly inhibited the proliferation and colony formation ability and induced cycle arrest at G2/M phase of AGS cells. IARS2 knockdown significantly decreased the expression levels of phosphorylation of (p-Smad2), p-SAPK/JUK, cleavage-Caspase-7, and p-TAK1, but increased the expression levels of p-53 and cleavage-PARP in AGS cells compared to shCtrl group. We demonstrated that IARS2...Continue Reading
References
Nov 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·J P KushnerS Dickman
Aug 30, 2001·Oncogene·M MiyakiT Iwama
Jul 11, 2003·Anti-cancer Drugs·Ryan W DellingerSaskia T C Neuteboom
Aug 3, 2004·Journal of Cell Science·Sang Won LeeSunghoon Kim
Oct 30, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chiun Chei LiHung Chiang
Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sang Gyu ParkSunghoon Kim
Sep 6, 2005·Cancer Research·Kosei ItoYoshiaki Ito
Apr 28, 2012·PloS One·Yijun WuHonglei Weng
May 30, 2012·Oncogene·C FreudlspergerC Van Waes
Sep 7, 2012·PloS One·Yong-Wan KimSunghoon Kim
Mar 1, 2011·Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu·Wan-Qing ChenPing Zhao
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Apr 16, 2014·Clinical Neurology and Neurosurgery·Paul MazarisSteven Kalkanis
Dec 8, 2015·Neoplasma·J YinK Wang
Dec 3, 2016·Nucleic Acids Research·Judith A BlakeUNKNOWN the Mouse Genome Database Group