Knockdown of lncRNA-PANDAR suppresses the proliferation, cell cycle and promotes apoptosis in thyroid cancer cells

EXCLI Journal
Zhirong LiDonglin Luo

Abstract

Long non-coding RNAs (lncRNAs) have been found to show important regulatory roles in various human cancers. Lnc-RNA PANDAR is a novel identified lncRNA that was previously reported to show abnormal expression pattern in various cancers. However, little is known of its expression and biological function in thyroid cancer. Here, we used the quantitative real-time PCR (qRT-PCR) to determine the expression of PANDAR in 64 thyroid cancer tissues. We found that expression of PANDAR was up-regulated in thyroid cancer tissues compared with adjacent non-tumor tissues. Functional assays in vitro demonstrated that knockdown of PANDAR could inhibit proliferation, cell cycle progression, induces the apoptosis, inhibit invasion of thyroid cancer cells. Thus, our study provides evidence that PANDAR may function as a potential target for treatment for patients with thyroid cancer.

Citations

Feb 26, 2019·Oncology Research and Treatment·Mohammad-Reza Mahmoudian-SaniGholam-Reza Mobini
Nov 14, 2019·Genes·Berta Luzón-ToroSalud Borrego
Dec 18, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri

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Methods Mentioned

BETA
transfection
flow cytometry
PCR

Software Mentioned

ImageJ
PANDAR
Cell Quest
SPSS

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