Knockdown of long non-coding RNA ANRIL inhibits tumorigenesis in human gastric cancer cells via microRNA-99a-mediated down-regulation of BMI1.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Pei LiuXiangjun Jiang

Abstract

Long non-coding RNA antisense non-coding RNA in the INK4 locus (ANRIL) has been reported to promote tumorigenesis via regulating microRNA (miR)-99a in gastric cancer cells. However, the role of each component involved in it is still not well understood. This study aimed to verify the role of ANRIL in gastric cancer as well as the underlying mechanisms. ANRIL levels in clinical gastric cancer tissues and cell lines were tested by qPCR. Effects of ANRIL silence on cell viability, migration and invasion, apoptosis, and miR-99a expression in MKN-45 and SGC-7901 cells were measured using CCK-8, Transwell assay, flow cytometry, and qPCR assays, respectively. Then, effects of miR-99a inhibition on ANRIL-silenced cells were evaluated. B-lymphoma Mo-MLV insertion region 1 (BMI1) expression, after abnormal expression of ANRIL and miR-99a, was determined. Finally, expression of key proteins in the apoptotic, Notch, and mTOR pathways was assessed. ANRIL level was elevated in gastric cancer tissues and cell lines. Knockdown of ANRIL suppressed cell viability, migration, and invasion, and increased apoptosis through up-regulating miR-99a. Furthermore, ANRIL silence down-regulated BMI1 via up-regulating miR-99a. BMI1 silence down-regulated Bc...Continue Reading

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Citations

Oct 24, 2019·Pathology Oncology Research : POR·Jun FangXiang-Ling Meng
Sep 29, 2020·Future Oncology·Ning LouYunbao Pan
Nov 25, 2020·Wiley Interdisciplinary Reviews. RNA·Joseph Eniafe, Shuai Jiang
Oct 5, 2020·Life Sciences·Sadegh FattahiHaleh Akhavan-Niaki
Jan 4, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-Fard, Mohammad Taheri
Dec 10, 2020·Cancers·Subhasree KumarJean-Pierre Etchegaray
Mar 21, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Nov 12, 2021·Frontiers in Oncology·Guangbing XiongRenyi Qin
Feb 23, 2020·The Journal of Surgical Research·Ryo SaitoDaisuke Ichikawa

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Datasets Mentioned

BETA
GES-1

Methods Mentioned

BETA
surgical resection
PCR
transfections
flow cytometry
protein assay
transfection

Software Mentioned

FlowJo
GraphPad Prism
GraphPad
Image Lab TM

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