Knockdown of long non-coding RNA TP73-AS1 inhibits cell proliferation and induces apoptosis in esophageal squamous cell carcinoma

Oncotarget
Wen-Qiao ZangGuoqiang Zhao

Abstract

Recent studies have shown that long non-coding RNAs (lncRNAs) are involved in a variety of biological processes and diseases in humans, including cancer. Our study serves as the first comprehensive analysis of lncRNA TP73-AS1 in esophageal cancer. We utilized a lncRNA microarray to analyze the expression profile of lncRNAs in esophageal squamous cell carcinoma. Our results show that lncRNA TP73-AS1 and BDH2 levels are generally upregulated in esophageal cancer tissues and are strongly correlated with tumor location or TNM stage in clinical samples. LncRNA TP73-AS1 knockdown inhibited BDH2 expression in EC9706 and KYSE30 cells, whereas BDH2 knockdown repressed esophageal cancer cell proliferation and induced apoptosis via the caspase-3 dependent apoptotic pathway. Overexpression of BDH2 in lncRNA TP73-AS1 knockdown cells partially rescued cell proliferation rates and suppressed apoptosis. In mouse xenografts, tumor size was reduced in lncRNA TP73-ASI siRNA-transfected tumors, suggesting that downregulation of lncRNA TP73-AS1 attenuated EC proliferation in vitro and in vivo. In addition, BDH2 or lncRNA TP73-AS1 knockdown enhanced the chemosensitivity of esophageal cancer cells to 5-FU and cisplatin. Our results suggest that lncRN...Continue Reading

References

Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Apr 24, 2002·Immunity·Oliver T Fackler, Andreas S Baur
May 6, 2003·Biochemical and Biophysical Research Communications·Luca Scorrano, Stanley J Korsmeyer
Dec 6, 2003·The New England Journal of Medicine·Peter C Enzinger, Robert J Mayer
Apr 20, 2004·Toxicology Letters·Sten Orrenius
Jun 11, 2004·Annual Review of Biochemistry·Xuejun Jiang, Xiaodong Wang
Nov 13, 2004·Circulation Research·Michael T CrowRichard N Kitsis
Oct 27, 2005·Journal of Biochemistry and Molecular Biology·Ssang-Goo Cho, Eui-Ju Choi
Feb 26, 2009·Cell·Chris P PontingWolf Reik
Jul 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ahmad M KhalilJohn L Rinn
Aug 8, 2009·Annual Review of Genetics·Chunxin Wang, Richard J Youle
Sep 5, 2009·Journal of Cancer Research and Clinical Oncology·Dan HuangXiang Du
May 25, 2010·Biochemical and Biophysical Research Communications·Erik NorbergBoris Zhivotovsky
Apr 15, 2011·Molecular Cancer·Ewan A GibbWan L Lam
Jun 12, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zu-Yau Lin, Wan-Long Chuang
Nov 28, 2014·Oncotarget·Federico Di GesualdoMatteo Lulli
Dec 30, 2014·The New England Journal of Medicine·Anil K Rustgi, Hashem B El-Serag
Jan 16, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Hui YuXia Meng
Jan 20, 2015·Nature Genetics·Matthew K IyerArul M Chinnaiyan
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Apr 4, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xue-Jiao ZhangFeng-Ying Huang
Apr 29, 2015·Journal of Surgical Oncology·Xuelin ZhangChengchu Zhu
Jun 18, 2015·Molecular and Cellular Therapies·Roshan FatimaSatyanarayana Mr Rao

❮ Previous
Next ❯

Citations

May 5, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Esra BaytakCan Küçük
Apr 14, 2017·Journal of Experimental & Clinical Cancer Research : CR·Shaling LiXue-Gong Fan
Jun 24, 2017·Journal of Cellular Biochemistry·Jia YaoEnxiang Zhou
Sep 15, 2018·Journal of Cellular Biochemistry·Xi ChenYuehong Liu
Dec 14, 2018·Bioscience Reports·Chunfeng LiuSongping Wang
Apr 3, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chengliang YangYufei Lu
May 16, 2020·Cancer Biotherapy & Radiopharmaceuticals·Zhongtao YuanWei Wang
Nov 23, 2019·World Journal of Gastroenterology : WJG·Feng-Zhi Li, Wen-Qiao Zang
Oct 28, 2019·Bioscience Reports·Caizhi ChenWen Zou
Jul 2, 2020·Journal of Experimental & Clinical Cancer Research : CR·Jia-Zhou LiuWan-Jiang Xue
Feb 9, 2017·Journal of Cancer Research and Clinical Oncology·Jia-Bin WangZhi-Guo Lin
Dec 12, 2018·Journal of Cellular Physiology·Rasoul AbdollahzadehJavad Tavakkoly-Bazzaz
Sep 25, 2017·Scientific Reports·Ami Ashar-PatelMelissa J Moore
Dec 26, 2018·Cancers·Ryte RynkevicieneKestutis Suziedelis
May 4, 2017·Cell Proliferation·Yang YuLin Miao
Jan 20, 2021·Cellular and Molecular Life Sciences : CMLS·Siyuan LuanYong Yuan
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhenzhang ChenDelin Liu
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tahereh FarkhondehSaeed Samarghandian
Jun 26, 2021·Pathology, Research and Practice·Seyed Hamed JafariMajid Nejati
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guangling YangXuejian Wu

❮ Previous
Next ❯

Datasets Mentioned

BETA
KYSE30

Methods Mentioned

BETA
transfection
flow cytometry
xenograft
PCR
Feature Extraction
Protein Assay
electrophoresis

Software Mentioned

SPSS
Living Image 3D
Feature Extraction
Cell Quest
Gene Spring

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis