Knockdown of Long Noncoding RNA ENST457720 Inhibits Proliferation of Non-Small Cell Lung Cancer Cells In Vitro and In Vivo.

Oncology Research
Jia YuShuyan Meng

Abstract

Non-small cell lung cancer (NSCLC) represents the leading cause of cancer-related mortality worldwide. More and more reports have identified important roles for long noncoding RNAs (lncRNAs) in cancer development. ENST457720 expression was upregulated in lung adenocarcinoma in a microarray-based lncRNA screen. We determined the expression levels of ENST457720 in NSCLC tissues with quantitative real-time PCR and then studied their clinical significance. We explored the biological significance of ENST457720 with gain- and loss-of-function analyses in vitro and in vivo. In this study, ENST457720 was expressed at higher levels in NSCLC tissues than in paired normal tissues. Higher ENST457720 expression was associated with larger tumor sizes, lymph node metastasis, and advanced TNM stage. ENST457720 silencing suppressed NSCLC cell proliferation in vitro and in vivo. Moreover, ENST457720 knockdown inhibited NSCLC invasion and reversed the epithelial-to-mesenchymal transition. ENST457720 promoted NSCLC proliferation and invasion, which may be a novel potential therapeutic target for NSCLC.

References

Aug 24, 2007·The Lancet Oncology·Arduino VerdecchiaUNKNOWN EUROCARE-4 Working Group
Aug 19, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jie LuoDi Zheng
Dec 20, 2014·Cancer Research·Maria V BogachekRonald J Weigel
Mar 12, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ping WangHongju Mao
Jul 15, 2015·Scientific Reports·Ying WangZhao-Qian Liu
Apr 26, 2016·Nature Structural & Molecular Biology·Youyou ZhangLin Zhang
Jun 18, 2016·Cell·Maninjay K AtianandKatherine A Fitzgerald
Jul 4, 2016·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Sacha I RothschildMario P Tschan

❮ Previous
Next ❯

Citations

Oct 20, 2018·Postgraduate Medical Journal·Shui-Ping DaiWei-Min Li
Apr 4, 2021·International Journal of Molecular Sciences·Anqing WuGuangming Zhou

❮ Previous
Next ❯

Methods Mentioned

BETA
transfections
transfection
Xenograft
flow cytometry

Software Mentioned

LNCipedia
SPSS
Ensembl

Related Concepts