Knockdown of Long Noncoding RNA LUCAT1 Inhibits Cell Viability and Invasion by Regulating miR-375 in Glioma.

Oncology Research
Yan-Sheng GaoLing-Zhen Li

Abstract

Recently, long noncoding RNAs (lncRNAs) have emerged as new gene regulators and prognostic markers in several cancers, including glioma. Here we focused on lncRNA LUCAT1 on the progression of glioma. qRT-PCR was used to determine the expression of LUCAT1 and miR-375 in glioma tissues and cells. MTT and Transwell invasion assays were performed to determine the function of LUCAT1 in glioma progression. The bioinformatics tool DIANA was used to predict the targets of LUCAT1. Pearson's correlation analysis was performed to explore the correlation between LUCAT1 and miR-375. In the present study, we showed that LUCAT1 was substantially upregulated in glioma tissues and cells. LUCAT1 inhibition significantly suppressed the proliferation and invasion of glioma cells. Subsequently, DIANA showed that miR-375 was predicted to contain the complementary binding sites to LUCAT1. Luciferase reporter assay showed that miR-375 directly targeted LUCAT1. In addition, we found that miR-375 was downregulated in glioma tissues and negatively correlated with LUCAT1 expression in glioma tissues. Furthermore, the results showed that miR-375 could rescue the function of LUCAT1 in glioma progression. The lncRNA LUCAT1 was critical for the proliferation ...Continue Reading

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Citations

Dec 28, 2018·Journal of Cellular and Molecular Medicine·Yun LouGuoqiang Li
Mar 5, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Liang ZhangHai-Rong Yao
Aug 20, 2020·Technology in Cancer Research & Treatment·Zhaoyu LiuYongheng Yang
Feb 12, 2020·Cellular & Molecular Biology Letters·Di WangErdong Chen
Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ce XingFeng Bai
Jan 8, 2021·Journal of Experimental & Clinical Cancer Research : CR·Xiangdong XuYiquan Ke
Mar 22, 2021·Life Sciences·Yang LiuChaolai Man
Apr 1, 2021·Journal of Cellular and Molecular Medicine·Kai ZhangZuode Gong
Sep 9, 2021·Bioscience Reports·Jiahui WeiJiabao Zhang
Jun 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuan YuanHe Xin

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Methods Mentioned

BETA
transfection

Software Mentioned

DIANA
SPSS17

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