Knockdown of melatonin receptor 1 and induction of follicle-stimulating hormone on the regulation of mouse granulosa cell function

Reproductive Biology
Hira Sajjad TalpurLi-Guo Yang

Abstract

Melatonin receptor 1 (MT1) performs a critical role in the regulation of the animal reproductive system, particularly in follicular growth, and has a considerable effect on reproductive performance. However, the role that MT1 plays in regulating hormones associated with reproduction remains unclear. This study was designed to examine the physiological role of constitutive MT1 silencing and follicle stimulating hormone (FSH) treatment in reproduction, making use of mouse granulosa cells (mGCs) as a model. To understand the constitutive role of MT1 in ovarian physiology, the RNAi-Ready pSIREN-RETROQ-ZsGreen Vector mediated recombinant pshRNA was used to silence MT1 gene expression. Furthermore, we observed that the expression of MT1 was successfully inhibited both at the protein and mRNA levels (P<0.001). We demonstrated that RNAi-B-mediated MT1 down-regulation significantly promoted apoptosis (P<0.001), inhibited proliferation, and regulated the cell cycle at the S-phase; conversely, FSH treatment partially aided the apoptotic effect and improved proliferation but showed a significant effect at the S-phase of the cell cycle. Transitory knockdown of MT1 proved essential in the function of mGCs, as it significantly decreased cycli...Continue Reading

Citations

Feb 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi FangYixun Liu
Feb 23, 2019·Animal Science Journal = Nihon Chikusan Gakkaihō·Hasan RiazLiguo Yang
Aug 4, 2021·Acta Biochimica Et Biophysica Sinica·Liyuan CuiMeirong Du
Nov 5, 2021·Reproductive Sciences·Baoqiang FuCheng Zhang

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