Knockdown of microRNA-29a Changes the Expression of Heat Shock Proteins in Breast Carcinoma MCF-7 Cells.

Oncology Research
Encieh ChoghaeiZahra Sadat Hashemi

Abstract

Breast cancer is the most commonly occurring cancer among women. MicroRNAs as noncoding small RNA molecules play pivotal roles in cancer-related biological processes. Increased levels of microRNA-29a in the serum of breast cancer patients have been reported. Since heat shock proteins (HSPs) play important roles in cell events, the quantitative fluctuations in their cellular levels could be deemed as key indicators of how the exerted treatment alters cell behavior. In this regard, using an antisense small RNA, we attempted to investigate the effects of miR-29a knockdown on the expression of HSPs genes in the MCF-7 breast cancer cell line. MCF-7 cells were cultured in high-glucose Dulbecco's modified Eagle's medium with 10% FBS. Studied cells were subdivided into five groups: treated with scramble, anti-miR-29a, anti-miR-29a + Taxol, Taxol, and control. Taxol was added 24 h post-anti-miR transfection and RNA extraction, and cDNA synthesis was done 48 h later. The changes in expression of HSP27, HSP40, HSP60, HSP70, and HSP90 were evaluated by real-time PCR. Our results revealed that inhibitors of microRNA-29a promote apoptosis through upregulation of HSP60 level and downregulation of HSP27, HSP40, HSP70, and HSP90 levels and coul...Continue Reading

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Citations

Aug 25, 2016·Biochimica Et Biophysica Acta·Yao-Fei PeiXi-Qiang Liu
Dec 9, 2017·Bioscience Reports·Jin-Yan WangJin-Hai Tang
Feb 13, 2018·Molecular and Clinical Oncology·Gholamreza KhamisipourShakib Shamsian
Sep 29, 2019·International Journal of Molecular Sciences·Hyun Seung BanHyun-Soo Cho
Jun 1, 2017·Antioxidants & Redox Signaling·Verónica MiguelSantiago Lamas
Sep 8, 2018·International Journal of Molecular Sciences·Celeste Caruso BavisottoClaudia Campanella
Mar 17, 2021·Journal of Cancer Research and Therapeutics·Salma M AbdelnasserMohsen M S Asker
May 22, 2021·Biomedical Materials·Saeideh Jafarinejad-FarsangiMariam Shahrokhi-Farjah
Aug 3, 2021·BioMed Research International·Mona G AlharbiMosleh M Abomughaid

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Methods Mentioned

BETA
Transfection
PCR

Software Mentioned

SPSS16

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