Knockdown of myosin VI by lentivirus-mediated short hairpin RNA suppresses proliferation of melanoma

Molecular Medicine Reports
Hui LiSen Yang

Abstract

Myosin VI has been reported to be associated with the progression of ovarian and prostate cancer. The aim of the present study was to reveal the role of myosin VI in the proliferation of melanoma. Briefly, lentivirus‑mediated short hairpin RNA (shRNA) was designed specifically to silence myosin VI in A375 and A431 cell lines. Expression levels of myosin VI were then analyzed in the two cell lines by quantitative polymerase chain reaction and western blot analyses. Cell viability was assessed using MTT and colony formation assays. In addition, the cell cycle distribution was determined by flow cytometry. The results demonstrated that knockdown of myosin VI significantly suppressed melanoma cell viability and proliferation, and induced cell cycle arrest in G0/G1 phase. To the best of our knowledge, the present study was the first to assess the role of myosin VI in the growth of melanoma. Knowledge of the underlying mechanism of the role myosin VI in skin cancer cells may aid in the development of novel methods of melanoma diagnosis and therapy in the future.

References

Jan 1, 1976·Cancer·M H ShiuJ G Fortner
Jun 1, 1986·International Journal of Radiation Oncology, Biology, Physics·J Overgaard
Oct 16, 1999·Nature·A L WellsH L Sweeney
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·R S RockH L Sweeney
Jan 5, 2002·Pigment Cell Research·R Sarangarajan, R E Boissy
Jan 19, 2002·Biochemical and Biophysical Research Communications·Abida AwanPeter L Stern
Jul 23, 2002·Nature Cell Biology·Erika R Geisbrecht, Denise J Montell
Oct 31, 2002·Seminars in Oncology·Emilio BajettaDavid Khayat
Jun 11, 2003·Nature Genetics·Alan J BridgeRichard Iggo
Mar 3, 2004·Nature Biotechnology·Charles E Samuel
May 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroyuki YoshidaHonami Naora
Apr 20, 2005·The Journal of Cell Biology·Daniela A SahlenderFolma Buss
Mar 2, 2006·Molecular and Cellular Biology·Eun Joo JungXinbin Chen
Sep 9, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Olivier ter BrakeBen Berkhout
Oct 31, 2006·The American Journal of Pathology·Thomas A DunnJun Luo
Dec 11, 2007·Biochemical and Biophysical Research Communications·Folma Buss, John Kendrick-Jones
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Feb 7, 2013·CA: a Cancer Journal for Clinicians·Carol DeSantisAhmedin Jemal

❮ Previous
Next ❯

Citations

Jan 21, 2021·Cancer Metastasis Reviews·Carmen Ruggiero, Enzo Lalli
Nov 12, 2020·Biology·Jaya Aseervatham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.