Knockdown of PAK1 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer Cells Through the ERK Pathway.

Applied Immunohistochemistry & Molecular Morphology : AIMM
Si WangYang Liu

Abstract

The p21-activated kinase (PAK) family of serine/threonine kinases plays a pivotal role in various human tumors, as supported by our previous report on the overexpressed PAK isoforms in non-small cell lung cancer (NSCLC). To better understand the role of PAKs in tumorigenesis, the authors examined PAK1 expression patterns and its significance in NSCLC. It was demonstrated by immunohistochemical staining that PAK1 was increased and localized in the cytoplasm in 151 of 207 cases. High levels of PAK1 expression correlated with a histologic type of tumor (squamous cell carcinoma), tumor node metastasis stage, and lymph nodal status. We also examined the biological role of PAK1 in lung cancer cell lines transfected with PAK1-small interfering RNA. Decreased expression of PAK1 inhibited lung cancer cell proliferation and invasion, which is the major cause of lung cancer malignancy. Downregulated expression of PAK1 hampered rapidly accelerated fibrosarcoma/mitogen-activated extracellular signal-regulated kinase/extracellular signal-regulated kinase pathway activity but did not affect Wnt/β-catenin signaling. Our findings suggest that PAK1 is an important oncogene in NSCLC, as decreased expression of PAK1 inhibited the proliferation and...Continue Reading

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Citations

Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jae Heun ChungDo Hyung Kim
Apr 8, 2021·Cancer Chemotherapy and Pharmacology·Min LiuZhicheng Gong
May 25, 2021·The Journal of International Medical Research·Chao-Qun Lin, Lu-Kui Chen
Aug 20, 2021·Applied Immunohistochemistry & Molecular Morphology : AIMM·Wei-Wei LiuSi Wang

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Methods Mentioned

BETA
Transfection
electrophoresis
Polymerase Chain Reaction
PCR

Software Mentioned

SPSS

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