Knockdown of peroxisome proliferator-activated receptor-beta induces less differentiation and enhances cell-fibronectin adhesion of colon cancer cells

Oncogene
L YangX-F Sun

Abstract

The role of peroxisome proliferator-activated receptor-beta/delta (PPAR-beta/delta) in the pathogenesis of colon cancer remains highly controversial. This study specifically silenced the PPAR-beta expression in three colon cancer cell lines with different metastatic potentials. Although PPAR-beta knockdown resulted in more malignant morphological changes, bigger colony sizes and lower carcinoembryonic antigen (CEA) secretion, and enhanced the cell-fibronectin adhesion, cell invasion and migration were unaffected. These effects were stronger in poorly metastatic cell lines compared with highly metastatic ones. Simultaneously, PPAR-beta knockdown decreased the mRNAs encoding adipocyte differentiation-related protein and liver fatty acid binding protein, and increased the mRNA of ILK, whereas the mRNAs encoding integrin-beta1 and angiopoietin-like 4 were unchanged. Using immunohistochemistry, we determined that the intensity of PPAR-beta expression was stronger in rectal cancers with better differentiation than in those with poor differentiation, and was stronger in early-stage tumors than in advanced ones. Together, these findings consistently indicate that PPAR-beta may facilitate differentiation and inhibit the cell-fibronectin...Continue Reading

References

Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·R A GuptaR N DuBois
May 17, 2001·Molecular and Cellular Biochemistry·F SchroederA B Kier
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yaacov BarakRonald M Evans
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Michael BordonaroAlan C Sartorelli
Mar 30, 2004·Nature Medicine·Fred S HarmanJeffrey M Peters
Apr 23, 2004·The Journal of Investigative Dermatology·Matthias SchmuthKenneth R Feingold
May 13, 2004·British Journal of Cancer·L C LawrieG I Murray
Jun 2, 2004·International Journal of Cancer. Journal International Du Cancer·Jordi CampsRosa Miró
Jul 13, 2004·Biochimica Et Biophysica Acta·Michele A WozniakPatricia J Keely
Oct 14, 2004·Oncogene·Karen R ReedAlan R Clarke
Jan 5, 2005·Nature Reviews. Cancer·Gregory HanniganShoukat Dedhar
Mar 22, 2005·Biochimica Et Biophysica Acta·Paul Targett-AdamsJohn McLauchlan
Jul 16, 2005·Cell Death and Differentiation·D J KimJ M Peters
Nov 1, 2005·Cancer Metastasis Reviews·Peter J Reddig, Rudy L Juliano
Dec 3, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Marjorie A PerazaJeffrey M Peters
Dec 20, 2005·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·L YangC Tian
Dec 28, 2005·Biochemical and Biophysical Research Communications·Cho S AungSarah J Roberts-Thomson
Mar 3, 2006·The Journal of Clinical Investigation·Grant D BarishRonald M Evans
May 23, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sadao TakahashiJuro Sakai
Oct 28, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Laura PiquerasDavid Bishop-Bailey
Nov 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ariane GalaupStephane Germain
May 20, 2008·The International Journal of Biochemistry & Cell Biology·Daniel C Worth, Maddy Parsons
Aug 19, 2008·Biochemistry. Biokhimii︠a︡·A Y Alexandrova

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Citations

Oct 2, 2012·PPAR Research·Joo-In Park, Jong-Young Kwak
Jul 9, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaogang JiangShemin Lu
Oct 3, 2012·PPAR Research·Valerio PazienzaGianluigi Mazzoccoli
Jul 6, 2014·Physiological Reviews·Jaap G Neels, Paul A Grimaldi
Oct 28, 2010·Molecular Cancer·Wenjing RuanMaode Lai
May 3, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lie YangXiao-Feng Sun
Dec 1, 2017·Cancer Research·Natalia Martín-MartínArkaitz Carracedo

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