Knockdown of Rab7a suppresses the proliferation, migration, and xenograft tumor growth of breast cancer cells

Bioscience Reports
Jiming XieYuzhen Wang

Abstract

Breast cancer is a common invasive cancer in women. Ras-related protein Rab-7a (Rab7a) is involved in late endocytic trafficking, while its role in breast cancer is largely unclear. In the present study, we investigated the role of Rab7a in breast cancer. Comparing with adjacent breast tissues, Rab7a expression was increased in breast cancer tissues. Using lentivirus-mediated knockdown strategy, we found that Rab7a silencing inhibited the proliferation and colony formation of MDA-MB-231 cells. Apoptosis and G2 cell cycle arrest were induced in Rab7a knockdown. By contrast, Rab7a suppressed the apoptosis and promoted proliferation and colony formation of MCF-7 cells. The migration of MDA-MB-231 cells was suppressed by Rab7a knockdown. In vivo, depletion of Rab7a inhibited the xenograft tumor development of MDA-MB-231 cells. Altogether, our results highlight the novel function of Rab7a in the proliferation, invasion, and xenograft tumor development of breast cancer cells.

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Citations

Jul 19, 2019·Medicinal Research Reviews·MeiHua WanTony Y Hu
Apr 3, 2020·Cold Spring Harbor Molecular Case Studies·Cora A RickerCharles Keller
Aug 23, 2020·International Journal of Molecular Sciences·Giuseppina AugimeriStefania Catalano
Feb 27, 2021·Drug Design, Development and Therapy·Sobhi M GomhaHuda K Mahmoud
Aug 20, 2020·Genomics·Hanchu XiongYongshi Jia
Aug 17, 2021·Computational and Mathematical Methods in Medicine·Ming CaoQinke Peng
Sep 14, 2021·Molecular Medicine Reports·Long HeDaifa Huang

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Methods Mentioned

BETA
PCR
fluorescence microscopy
flow cytometry
xenograft

Software Mentioned

GraphPad Prism
IPA

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