Knockdown of RAC1 and VASP gene expression inhibits breast cancer cell migration

Oncology Letters
Yihao TianLei Wei

Abstract

The ability of tumor cells to migrate is biologically fundamental for tumorigenesis, growth, metastasis and invasion. The present study examined the role of Ras-related C3 botulinum toxin substrate (RAC1) and vasodilator-stimulated phosphoprotein (VASP) in breast cancer cell migration. According to data in Kaplan, Oncomine and The Cancer Genome Atlas, increased expression levels of RAC1 and VASP in breast cancer are associated with decreased cancer cell differentiation, advanced pathological stage and more aggressive tumor subtypes, while increased VASP mRNA expression levels are positively correlated with a poor prognosis in patients with breast cancer. The short hairpin (sh)RNA technique was employed to knock down the expression of RAC1 or VASP. Stable interference with the expression of RAC1 or VASP using RAC1-shRNA or VASP-shRNA, respectively, was established in MCF-7 breast cancer cells. In RAC1-shRNA or VASP-shRNA cells, the protein expression levels of RAC1 or VASP were significantly downregulated compared with control cells. The proliferation and migration rates of the RAC1-shRNA or VASP-shRNA cells were significantly lower compared with control cells. It was observed that the protein expression levels of VASP also decr...Continue Reading

References

Feb 7, 1998·Science·A Hall
Apr 17, 1999·Molecular and Cellular Biology·K LiuS N Cohen
Nov 1, 2000·Cell·D Bar-Sagi, A Hall
Mar 15, 2001·Cancer Letters·S Aznar, J C Lacal
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Matthias KrauseFrank B Gertler
Feb 24, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Antonio S Sechi, Juergen Wehland
Jan 1, 2008·The American Journal of Pathology·Carolina EspinaJuan Carlos Lacal
May 2, 2008·The Journal of Biological Chemistry·Kim Buay LimSohail Ahmed
May 21, 2008·Handbook of Experimental Pharmacology·G Pula, M Krause
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Joel S ParkerPhilip S Bernard
Apr 19, 2011·European Journal of Pharmacology·Rebeca López-MarureJoseph S Dillon
Jun 28, 2011·International Journal of Cancer. Journal International Du Cancer·David S ZuzgaGiovanni M Pitari
Jul 19, 2014·Small GTPases·Jose L OrgazVictoria Sanz-Moreno
Dec 9, 2014·Small GTPases·Antje SchaeferPeter L Hordijk
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Dec 20, 2016·Oncogene·I ZandvakiliY Zheng
Jul 3, 2017·Biochemical and Biophysical Research Communications·Yu-Long LiDe-Li Shi
Aug 16, 2017·Cancer Research·Marcelo G Kazanietz, Maria J Caloca
Aug 30, 2017·International Journal of Oncology·G A CardamaD E Gomez

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Citations

Apr 22, 2020·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Mio YamaguchiTakashi Suzuki
Nov 12, 2020·Biology·Jaya Aseervatham

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Methods Mentioned

BETA
GTPases
fluorescence microscopy
motility assays
flow cytometry
Assay
transfection

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