Knockdown of Ran GTPase expression inhibits the proliferation and migration of breast cancer cells

Molecular Medicine Reports
Chenyi ShengQichao Ni

Abstract

Breast cancer is the second leading cause of cancer‑associated mortality in women worldwide. Strong evidence has suggested that Ran, which is a small GTP binding protein involved in the transport of RNA and protein across the nucleus, may be a key cellular protein involved in the metastatic progression of cancer. The present study investigated Ran gene expression in breast cancer tissue samples obtained from 140 patients who had undergone surgical resection for breast cancer. Western blot analysis of Ran in breast cancer tissues and paired adjacent normal tissues showed that expression of Ran was significantly increased in breast cancer tissues. Immunohistochemistry analyses conducted on formalin‑fixed paraffin‑embedded breast cancer tissue sections revealed that Ran expression was associated with tumor histological grade, nerve invasion and metastasis, vascular metastasis and Ki‑67 expression (a marker of cell proliferation). Kaplan‑Meier survival analysis showed that increased Ran expression in patients with breast cancer was positively associated with a poor survival prognosis. Furthermore, in vitro experiments demonstrated that highly migratory MDA‑MB‑231 cancer cells treated with Ran‑si‑RNA (si‑Ran), which knocked down exp...Continue Reading

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Citations

Dec 23, 2019·The Journal of Cell Biology·Stephanie C Ems-McClungClaire E Walczak
Mar 20, 2019·Cells·Néstor Prieto-DominguezYong Teng
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Zied BoudhraaAnne-Marie Mes-Masson
Apr 4, 2021·Cancers·Paulo Matos

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Methods Mentioned

BETA
GTPase
surgical resection
transfection

Software Mentioned

Photoshop
Cell Quest
SPSS

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