Knockdown of the long noncoding RNA HOTTIP inhibits cell proliferation and enhances cell sensitivity to cisplatin by suppressing the Wnt/β-catenin pathway in prostate cancer

Journal of Cellular Biochemistry
Huichuan JiangYuan Li

Abstract

Prostate cancer (PCa) is a prevalent and deadly cancer worldwide. Considering the malignant progression and therapeutic resistance of PCa, further dissection of the underlying mechanisms and exploration of novel therapeutic targets for PCa are urgently needed. The long noncoding RNA HOTTIP has recently been revealed as an oncogenic regulator in different cancers; however, whether HOTTIP is involved in PCa remains poorly understood. Here, we examined the crucial roles of HOTTIP in the proliferation and chemoresistance of PCa. Quantitative real-time PCR (qRT-PCR) was performed to detect the HOTTIP messenger RNA (mRNA) levels in PCa samples from patients and PCa cells. Then, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation, and cell cycle and flow cytometry assays were performed to investigate the proliferation and cisplatin-resistance of PCa cells with silenced HOTTIP compared with a negative control. We applied Western blotting, qRT-PCR and a TOP/FOP assay to explore the relevant mechanisms. In this study, we found that the HOTTIP mRNA levels were increased in the PCa patient samples and PCa cell lines compared with the controls. The knockdown of HOTTIP not only inhibited the proliferation of ...Continue Reading

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Citations

Jun 7, 2020·International Journal of Molecular Sciences·Milad AshrafizadehGautam Sethi
Nov 30, 2019·International Journal of Molecular Sciences·Ti-Hui WuYa-Wen Lin
Jul 1, 2020·Cancer Metastasis Reviews·Yang PengYutaka Kondo
Oct 2, 2019·Pathology, Research and Practice·Yoo Jin LeeChul Hwan Kim
Nov 19, 2020·Seminars in Cancer Biology·Wangkai XieZhi-Wei Wang
Jun 10, 2021·Cell Death & Disease·Lifeng DingGonghui Li

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