PMID: 19148532Jan 17, 2009Paper

Knockdown of the novel proteasome subunit Adrm1 located on the 20q13 amplicon inhibits colorectal cancer cell migration, survival and tumorigenicity

Oncology Reports
Wei ChenChao He

Abstract

The novel proteasome subunit Adrm1 located on the 20q13 amplicon was differentially expressed in colorectal cancer by semiquantitative RT-PCR. Adrm1 mRNA was overexpressed in 46.2% (18/39) colorectal cancer tissues compared to their matched normal mucosa and significantly correlated with lymph node metastasis of colorectal cancer (P=0.037). Knockdown of Adrm1 by shRNA in human colon carcinoma RKO cells inhibited their anchorage-independent growth, cell migration as well as cell proliferation through inducing apoptosis and cell cycle arrest at the G1 phase. In addition, stable RNA interference of Adrm1 gene synergistic with 5-Fu treatment suppressed RKO cell growth in vitro. Collectively, these data suggested that Adrm1 is potentially oncogenic and may play an important role in colon tumorigenesis. Regiment with combined application of Adrm1 RNA interference and chemotherapy may emerge as a novel therapeutic strategy for Adrm1 overexpressed colorectal cancer.

Citations

Feb 5, 2013·International Journal of Molecular Sciences·Marlena S FejzoDennis J Slamon
Jun 28, 2014·Clinical & Experimental Metastasis·Seok Hoon JangHark Kyun Kim
Oct 13, 2009·Biochemical and Biophysical Research Communications·Taesoo KimYongwon Choi
Aug 13, 2009·Genes, Chromosomes & Cancer·James F ReidMarco A Pierotti
Mar 22, 2016·European Journal of Pharmacology·Karen BolandJochen H M Prehn
Dec 23, 2009·Journal of Proteomics·Huixing FengWei Ning Chen
Feb 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·David J Sherman, Jing Li
Sep 12, 2020·The Kaohsiung Journal of Medical Sciences·Yu-Cen LiangYu-Xia Liang

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