Knockdown of TIPE2 increases the proliferation in lipopolysaccharide-stimulated gastric cancer cells.

BMC Cancer
Wenming LiuZhongquan Qi

Abstract

Gastric cancer (GC) is one of the most common malignant diseases with high morbidity and mortality, especially in Asian countries. During the GC developing progress, TIPE2, a member of TNF-alpha induced protein 8-like (TNFAIP8L) family, may play important roles. However, the molecular mechanisms of TIPE2 contributing to cell proliferation and tumor growth are poorly understood in GC. We performed flow cytometry to detect the cell cycle of TIPE2-knockdown GC cells under lipopolysaccharide (LPS) stimulation. We measured TIPE2 expression in tumor samples from 46 human GC patients at mRNA level by Realtime PCR and in 68 pairs of GC tissues at protein level by immunohistochemistry. We established stable TIPE2 knockdown SGC7901 and BGC823 cell lines and performed CCK-8 and EdU proliferation assays under the stimulation of LPS. And then we analyzed AKT, IκBα and ERK phosphorylation levels, as well as cycle related proteins CDK4 and CyclinD3 in the stable TIPE2 knockdown SGC7901 and BGC823 cells. Our present studies indicated that the expression of TIPE2 was significantly decreased in tumor tissues compared to distant mucosa tissues in human GC patients. TIPE2 inhibited proliferation stimulated by LPS in SGC7901 and BGC823 cells. Silen...Continue Reading

References

Sep 20, 2011·Molecular Immunology·Yunwei Lou, Suxia Liu
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Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network
Sep 23, 2014·Cancer Cell·Svetlana A FayngertsYouhai H Chen
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Methods Mentioned

BETA
flow cytometry

Software Mentioned

GraphPad
ModFit
GraphPad Prism
SPSS

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