Knockdown of TRAP1 promotes cisplatin-induced apoptosis by promoting the ROS-dependent mitochondrial dysfunction in lung cancer cells.

Molecular and Cellular Biochemistry
Xiaowei ZhangHua Guo

Abstract

The tumor necrosis factor receptor-associated protein 1 (TRAP1) is associated with the occurrence and development of various diseases, including inflammation and cancer. However, the role and mechanism of TRAP1 in the development of lung cancer need to be further explored. Therefore, the purpose of this study is to investigate the role of TRAP1 in the regulation of apoptosis by cisplatin and its special mechanism. The RT-qPCR and Western blot were used to detect the mRNA and protein expression of ANGPTL4 in A549 and H1299 cells, respectively. And the cell apoptosis and cell cycle were measured by flow cytometry (FCM). The expression of genes related to apoptosis and drug resistance as well as the cell cycle regulators, including MDM2, CyclinB1, and CDK1, were detected by Western blot. Finally, the reactive oxygen species (ROS) indicator DCFH-DA was performed to detect the generation of ROS, and the mitochondrial membrane potential (ΔΨm) was detected by JC-1 staining. The results showed that the expression of TRAP1 was significantly increased in A549/DDP and H1299/DDP than A549 and H1299 cells. Further research found that knockdown of TRAP1 induced apoptosis and caused G2/M cell cycle arrest in A549/DDP and H1299/DDP cells. What...Continue Reading

References

Dec 2, 2000·Science·D J Klionsky, S D Emr
Jan 24, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hirohito TadaHarubumi Kato
Mar 15, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Badreldin H Ali, Mansour S Al Moundhri
Jun 19, 2008·Genes & Development·Der-Fen SuenRichard J Youle
Nov 3, 2009·Trends in Cell Biology·Daniel Speidel
Mar 13, 2010·The Journal of Pathology·Danielle GlickKay F Macleod
Sep 1, 2011·Biochimica Et Biophysica Acta·Dario C AltieriLucia R Languino
Jan 26, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Azhar RasulXiaomeng Li
Apr 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Soichiro YoshidaLeonard Neckers
Jul 12, 2013·Nature Communications·Young Chan ChaeDario C Altieri
Feb 26, 2014·Molecular Cancer Research : MCR·Jackeline AgorretaFrancesco Pezzella
Apr 12, 2014·Oncotarget·Emma ShtivelmanRavi Salgia
Jul 24, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peng ZhangWei Hu
Jul 16, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun-Feng ChenQing He

❮ Previous
Next ❯

Citations

May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sepideh MirzaeiKwang-Seok Ahn
Jul 6, 2021·Free Radical Biology & Medicine·Tomohiro NakamuraStuart A Lipton

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
transfection
transfecting

Software Mentioned

SPSS
ImageJ

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis