Knockdown of UbcH10 enhances the chemosensitivity of dual drug resistant breast cancer cells to epirubicin and docetaxel

International Journal of Molecular Sciences
Cheng WangLi-Ming Zhao

Abstract

Breast cancer is one of the most common and lethal cancers in women. As a hub gene involved in a diversity of tumors, the ubiquitin-conjugating enzyme H10 (UbcH10), may also play some roles in the genesis and development of breast cancer. In the current study, we found that the expression of UbcH10 was up-regulated in some breast cancer tissues and five cell lines. We established a dual drug resistant cell line MCF-7/EPB (epirubicin)/TXT (docetaxel) and a lentiviral system expressing UbcH10 shRNA to investigate the effects of UbcH10 knockdown on the chemosensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel. The knockdown of UbcH10 inhibited the proliferation of both MCF-7 and MCF-7/EPB/TXT cells, due to the G1 phase arrest in cell cycle. Furthermore, UbcH10 knockdown increased the sensitivity of MCF-7/EPB/TXT cells to epirubicin and docetaxel and promoted the apoptosis induced by these two drugs. Protein detection showed that, in addition to inhibiting the expression of Ki67 and cyclin D1, UbcH10 RNAi also impaired the increased BCL-2 and MDR-1 expression levels in MCF-7/EPB/TXT cells, which may contribute to abating the drug resistance in the breast cancer cells. Our research in the current study demonstrated that up...Continue Reading

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Citations

Oct 19, 2017·Cancer Biomarkers : Section a of Disease Markers·Si SunShengrong Sun
Mar 21, 2020·International Journal of Molecular Sciences·Ivan PrestaGiuseppe Donato
Jan 6, 2018·Cancer Biology & Therapy·Yan ZhangChang Liu
Sep 8, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tao QinMan Li
Jul 3, 2021·Cells·Quyen Thu BuiPeter Chang-Whan Lee

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Methods Mentioned

BETA
protein
PCR
Transfection
transfect
gene knockdown
fluorescence microscopy
flow cytometry
X-ray

Software Mentioned

SPSS13
SPSS

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