Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region.

Parasites & Vectors
Katrijn VerhaeghenMarc Coosemans

Abstract

In the Mekong region (Vietnam, Cambodia and Laos), a large investigation was conducted to assess the susceptibility of Anopheles species against DDT and pyrethroids. In this study, the resistance status of the potential malaria vectors An. vagus, An. sinensis, An. paraliae and An. peditaeniatus was assessed. Bioassays were performed on field collected unfed female mosquitoes using the standard WHO susceptibility tests. In addition, the DIIS6 region of the para-type sodium channel gene was amplified and sequenced and four allele-specific PCR assays were developed to assess the kdr frequencies. In Southern Vietnam all species were DDT and pyrethroid resistant, which might suggest the presence of a kdr resistance mechanism. Sequence-analysis of the DIIS6 region of the para-type sodium channel gene revealed the presence of a L1014S kdr mutation in An. vagus, An. sinensis and An. paraliae. In An. peditaeniatus, a low frequency L1014S kdr mutation was found in combination with a high frequency L1014F kdr mutation. For pyrethroids and DDT, no genotypic differentiation was found between survivors and non-survivors for any of these species. In the two widespread species, An. vagus and An. sinensis, kdr was found only in southern Vietnam...Continue Reading

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Citations

Aug 5, 2011·Parasites & Vectors·Yousif E HimeidanHilary Ranson
Jun 19, 2013·Parasites & Vectors·Guoding ZhuJetsumon Sattabongkot
Sep 11, 2013·Pesticide Biochemistry and Physiology·Frank D RinkevichKe Dong
Mar 9, 2011·Acta Tropica·Liwang CuiGuofa Zhou
Apr 19, 2015·Malaria Journal·Hong-wei ZhangBian-li Xu
Jan 10, 2017·Frontiers in Microbiology·Chung T NgoEstelle Jumas-Bilak
Apr 1, 2017·Parasites & Vectors·Victor ChaumeauVincent Corbel
Apr 25, 2017·PloS One·Sébastien MarcombePaul T Brey
Jan 11, 2012·The American Journal of Tropical Medicine and Hygiene·Cara N Henry-HalldinPeter A Zimmerman
Mar 18, 2015·The American Journal of Tropical Medicine and Hygiene·Moses R KamyaGrant Dorsey
Mar 17, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rada M SavicFrancesca T Aweeka
Mar 17, 2018·Frontiers in Public Health·Christine Ludwin Wanjala, Eliningaya J Kweka

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Methods Mentioned

BETA
ELISA
PCR
PCRs

Software Mentioned

GENEPOP
ClustalW

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