Knocking-down the expression of nucleostemin significantly decreases rate of proliferation of rat bone marrow stromal stem cells in an apparently p53-independent manner.

Cell Proliferation
S M JafarnejadM M Matin

Abstract

Nucleostemin (NS) is a recently identified GTP-binding protein, predominantly expressed in embryonic and adult stem cells but not in terminally differentiated cells. NS is expressed in bone marrow-derived mesenchymal stem cells, and its expression ceases upon induction of neural differentiation. The major aim of this study was to determine whether down-regulation of NS expression acts as a promoter, or otherwise as a by-product of differentiation and senescence processes. We used RNA interference protocols to specifically knock down NS in rat bone marrow-derived stromal stem cells. Changes in rate of proliferation and cell cycle profile after knocking-down of NS were measured. In addition, changes in expression of associated genes were studied by semiquantitative RT-PCR, Western blotting and immunocytochemistery. Knocked-down expression of NS caused a significant decrease in the rate of cell proliferation with concomitant shutting off of expression of cyclin D1 and survivin, two other well-known regulators of cell proliferation. Interestingly, we noticed no obvious changes in expression level of p21, the main effector of p53 for its cell cycle repressing function. Our findings revealed a master role for NS in promoting prolifer...Continue Reading

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Citations

Apr 17, 2010·Cell Biology and Toxicology·Modjtaba Emadi BaygiWolfgang A Schulz
May 28, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Modjtaba Emadi BaygiWolfgang A Schulz
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lingjun MengRobert Y L Tsai
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Jun 24, 2010·Molecular Carcinogenesis·Mahshid MalakootianAfsaneh Malekzadeh Shafaroudi
Feb 3, 2016·Cell Death Discovery·Guanqun HuangRobert Y L Tsai
Apr 26, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Malek Hossein AsadiSeyed Javad Mowla
Apr 11, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Yu GuoChang-Qing Liu
Aug 17, 2014·Journal of Cell Science·Robert Y L Tsai

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