Knockout of Apolipoprotein E in rabbit promotes premature intervertebral disc degeneration: A new in vivo model for therapeutic approaches of spinal disc disorders

PloS One
Anja BeierfußDemissew S Mern

Abstract

Intervertebral disc (IVD) degeneration that accelerates the loss of disc structural and functional integrities is recognized as one of the major factors of chronic back pain. Cardiovascular risk factors, such as deficits of apolipoproteins that elevate the levels of cholesterol and triglycerides, are considered critical for the progress of atherosclerosis; notably in the abdominal aorta and its lumbar branching arteries that supply lumbar vertebrae and IVDs. Obstruction of the lumbar arteries by atherosclerosis is presumed to promote lumbar disc degeneration and low back pain. APOE-knockout rabbits have recently been shown to generate hyperlipidemia with increased levels of cholesterol and triglycerides that mimic the symptoms of atherosclerosis in humans. Here, we analysed IVD degeneration in the lumbar spines of ten homozygous APOE-knockout and four wild-type New Zealand White rabbits of matching age to prove accelerated IVD degeneration in APOE-knockout rabbits, since APOE-knockout rabbits could be a beneficial model for therapeutic approaches of degenerative IVD disorders. Experiments were performed using T1/T2-weighted magnetic resonance imaging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, glucose-o...Continue Reading

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Citations

Nov 27, 2019·British Journal of Pharmacology·Ioanna AndreadouPéter Ferdinandy
May 16, 2019·The Kaohsiung Journal of Medical Sciences·Hua-Cong WangYong Liu
Aug 19, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wei WuZengwu Shao
Nov 12, 2020·Experimental Biology and Medicine·Demissew Shenegelegn MernClaudius Thomé
Jan 24, 2022·Cell Biology International·Jing-Hua TanJing-Bo Xue

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Methods Mentioned

BETA
FCS
Assay
Protein Assay
enzyme-linked immunosorbent assay
ELISA
reverse transcription PCR
PCR
electrophoresis

Software Mentioned

SPSS Statistics
ImageJ

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