Knockout of BRD7 results in impaired spermatogenesis and male infertility

Scientific Reports
Heran WangMing Zhou

Abstract

BRD7 was originally identified as a novel bromodomain gene and a potential transcriptional factor. BRD7 was found to be extensively expressed in multiple mouse tissues but was highly expressed in the testis. Furthermore, BRD7 was located in germ cells during multiple stages of spermatogenesis, ranging from the pachytene to the round spermatid stage. Homozygous knockout of BRD7 (BRD7(-/-)) resulted in complete male infertility and spermatogenesis defects, including deformed acrosomal formation, degenerative elongating spermatids and irregular head morphology in postmeiotic germ cells in the seminiferous epithelium, which led to the complete arrest of spermatogenesis at step 13. Moreover, a high ratio of apoptosis was determined by TUNEL analysis, which was supported by high levels of the apoptosis markers annexin V and p53 in knockout testes. Increased expression of the DNA damage maker λH2AX was also found in BRD7(-/-) mice, whereas DNA damage repair genes were down-regulated. Furthermore, no or lower expression of BRD7 was detected in the testes of azoospermia patients exhibiting spermatogenesis arrest than that in control group. These data demonstrate that BRD7 is involved in male infertility and spermatogenesis in mice, and ...Continue Reading

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Citations

Aug 25, 2017·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Yue-Qiu TanGe Lin
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Methods Mentioned

BETA
PCR
transmission electronic microscopy
biopsies
histones acetylation
Genotyping
electrophoresis
light microscopy
flow cytometry

Software Mentioned

QCapture Pro
SPSS

Related Concepts

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Apoptosis

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