Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality

Molecular and Cellular Endocrinology
Maria João MenesesMarco G Alves

Abstract

Insulin-degrading enzyme (IDE) is a zinc metalloprotease responsible for degrading and inactivating several bioactive peptides, including insulin. Individuals without this enzyme or with a loss-of-function mutation in the gene that codifies it, present hyperinsulinemia. In addition, impairment of IDE-mediated insulin clearance is associated with the development of metabolic diseases, namely prediabetes. Although insulin regulates male fertility, the role of IDE on male reproductive function remains unknown. We proposed to study the influence of IDE in the reproductive potential of males. As insulin mediates key events for the normal occurrence of spermatogenesis, we hypothesized that IDE functioning might be linked with sperm quality. We used C57BL/6N mice that were divided in three groups according to its genotype: wild type (WT), heterozygous and knockout (KO) male mice for Ide. Spermatozoa were collected from the cauda of epididymis and sperm parameters were evaluated. Testicular tissue morphology was assessed through hematoxylin and eosin stain. Mitochondrial complex protein levels and lipid peroxidation were also evaluated in the testicular tissue. Our results show that KO mice present a 50% decrease in testes weight compa...Continue Reading

Citations

Aug 20, 2019·Annals of Work Exposures and Health·Kyung-Hwa ChoiHo-Jang Kwon
Jan 23, 2021·Biomedicines·Carlos M González-CasimiroGermán Perdomo
Aug 10, 2021·Frontiers in Endocrinology·Wasim ShahXiaohua Jiang
Aug 17, 2021·The Journal of Pathology·Luís SousaHugo Vicente Miranda

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