Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain

Neural Plasticity
Gui-Lin JinChang-Xi Yu

Abstract

Despite decades of studies, the currently available drugs largely fail to control neuropathic pain. Koumine-an alkaloidal constituent derived from the medicinal plant Gelsemium elegans Benth.-has been shown to possess analgesic and anti-inflammatory properties; however, the underlying mechanisms remain unclear. In this study, we aimed to investigate the analgesic and anti-inflammatory effects and the possible underlying mechanisms of koumine. The analgesic and anti-inflammatory effects of koumine were explored by using chronic constriction injury of the sciatic nerve (CCI) neuropathic pain model in vivo and LPS-induced injury in microglia BV2 cells in vitro. Immunofluorescence staining and Western blot analysis were used to assess the modulator effect of koumine on microglia and astrocyte activation after CCI surgery. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the levels of proinflammatory cytokines. Western blot analysis and quantitative real-time polymerase chain reaction (qPCR) were used to examine the modulator effect of koumine on microglial M1 polarization. We found that single or repeated treatment of koumine can significantly reduce neuropathic pain after nerve injury. Moreover, koumine showed inhibi...Continue Reading

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Citations

Jul 10, 2019·Pain Research & Management : the Journal of the Canadian Pain Society = Journal De La Société Canadienne Pour Le Traitement De La Douleur·Jiang BianQing Liu
Mar 28, 2019·Journal of Immunology Research·Rongcai YueChangxi Yu
Oct 8, 2020·Chinese Medicine·Chunhao ZhuJianqiang Yu
May 23, 2020·Brain, Behavior, and Immunity·Katrien PletinckxTorsten R Dunkern
Aug 26, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xue-Jia QiZhao-Ying Liu

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Methods Mentioned

BETA
Protein Assay
ELISA

Software Mentioned

NIH ImageJ
Image
Pro Plus

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