KRAS, BRAF and PIK3CA mutations and the loss of PTEN expression in Chinese patients with colorectal cancer.

PloS One
Chen MaoQing Chen

Abstract

To investigate the frequency and relationship of the KRAS, BRAF and PIK3CA mutations and the loss of PTEN expression in Chinese patients with colorectal cancer (CRC). Genomic DNA was extracted from the formalin-fixed paraffin-embedded (FFPE) tissues of 69 patients with histologically confirmed CRC. Automated sequencing analysis was conducted to detect mutations in the KRAS (codons 12, 13, and 14), BRAF (codon 600) and PIK3CA (codons 542, 545 and 1047). PTEN protein expression was evaluated by immunohistochemistry on 3 mm FFPE tissue sections. Statistical analysis was carried out using SPSS 16.0 software. The frequency of KRAS, BRAF and PIK3CA mutations and loss of PTEN expression was 43.9% (25/57), 25.4% (15/59), 8.2% (5/61) and 47.8% (33/69), respectively. The most frequent mutation in KRAS, BRAF and PIK3CA was V14G (26.7% of all mutations), V600E (40.0% of all mutations) and V600L (40.0% of all mutations), and H1047L (80.0% of all mutations), respectively. Six KRAS mutant patients (24.0%) harbored BRAF mutations. BRAF and PIK3CA mutations were mutually exclusive. No significant correlation was observed between the four biomarkers and patients' characteristics. BRAF mutation rate is much higher in this study than in other stud...Continue Reading

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Citations

Oct 15, 2013·PloS One·Zhi-Jun SunAshok B Kulkarni
Jan 11, 2014·World Journal of Gastroenterology : WJG·Qi XueGuo-Xin Li
Apr 8, 2015·Critical Reviews in Food Science and Nutrition·Ulf HammerlingNils-Gunnar Ilbäck
Jun 18, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fangyuan JingKun Chen
Aug 2, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xu-Dong YangQin-Gang Hu
Aug 15, 2015·International Journal of Endocrinology·Li-Bo YangFeng Ye
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May 8, 2019·Indian Journal of Cancer·Ozturk Ates, Suayib Yalcin
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Jan 1, 2015·The Journal of Pathology. Clinical Research·Niek HugenIris D Nagtegaal
Jul 5, 2018·Cell Death & Disease·Qiang WangJingwang Bi

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Methods Mentioned

BETA
PCR
GTPases
GTPase

Software Mentioned

SPSS

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