KRAS, NRAS, BRAF mutations and high counts of poorly differentiated clusters of neoplastic cells in colorectal cancer: observational analysis of 175 cases

Pathology
Valeria BarresiStefania Bettelli

Abstract

A novel grading system based on the counting of poorly differentiated clusters (PDC) of neoplastic cells at the invasive margin and in the tumour stroma was recently introduced among the histological parameters predictive of adverse clinical outcome in colorectal cancer (CRC). The aim of this study was to correlate the histological grade based on PDC and the mutational status of KRAS, NRAS and BRAF genes in 175 consecutive CRCs. The highest PDC count under the objective lens of a ×20 microscopic field in each tumour was considered for grading assessment, so that PDC counts <5, 5-9 and ≥10 PDCs were defined grade 1, grade 2 and grade 3, respectively. Hotspots mutations were identified using the MassArray platform. Overall, there were 42 (24%) mutated tumours. Mutational status was significantly associated with high pT stage (p = 0.0021), advanced pTNM stage (p = 0.0018), nodal metastases (p = 0.006), tumour budding (p = 0.022) and high PDC grade (p = 0.0022). KRAS mutations were significantly associated with PDC grade (p = 0.0379), while BRAF mutations were associated with PDC-G3 although statistical significance was not reached. No significant associations were found between NRAS and PDC. The significant association between mut...Continue Reading

References

Nov 7, 2000·Journal of Surgical Oncology·T NozoeK Sugimachi
Nov 13, 2009·International Journal of Cancer. Journal International Du Cancer·Inti ZlobecAlessandro Lugli
Feb 22, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Montse VerdúXavier Puig
May 25, 2011·International Journal of Cancer. Journal International Du Cancer·Hermann BrennerUNKNOWN EUROCARE Working Group
Dec 8, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shuji OginoCharles S Fuchs
Jan 19, 2012·The American Journal of Surgical Pathology·Hideki UenoKazuo Hase
Oct 10, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Patrick G GavinKay L Pogue-Geile
Jan 26, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Christophe RostyDaniel D Buchanan
Jul 9, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Brian C Netzel, Stefan K G Grebe
Apr 29, 2014·Virchows Archiv : an International Journal of Pathology·Valeria BarresiGiovanni Tuccari
Jun 24, 2014·Cell·Diane D ShaoWilliam C Hahn
Jun 27, 2014·Journal of Surgical Oncology·Chun-Chi LinShih-Ching Chang
Aug 26, 2014·Human Pathology·Christophe RostyDaniel D Buchanan

❮ Previous
Next ❯

Citations

Mar 24, 2016·Diagnostic Pathology·Luca Reggiani BonettiCristian Palmiere
Jul 23, 2016·Clinical Colorectal Cancer·Valeria BarresiGiovanni Tuccari
Sep 5, 2019·European Journal of Cancer Care·Mahmood MoosazadehFereshteh Rostami-Maskopaee
Jul 19, 2019·The American Journal of Surgical Pathology·Keisuke YonemuraHideki Ueno
Sep 10, 2020·Nature Reviews. Clinical Oncology·Alessandro LugliIris D Nagtegaal
Jul 19, 2018·Disease Markers·Luca Reggiani BonettiStefania Bettelli
Mar 26, 2021·Nature Cancer·Jakob Nikolas KatherTom Luedde
Jul 25, 2021·Journal of Gastrointestinal Cancer·Kazumori AraiKo Ohata

❮ Previous
Next ❯

Software Mentioned

Typer
SPSS
Sequenom

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.