Krüppel homolog 1 and E93 mediate Juvenile hormone regulation of metamorphosis in the common bed bug, Cimex lectularius

Scientific Reports
Hemant Gujar, Subba R Palli

Abstract

The common bed bug is an obligate hematophagous parasite of humans. We studied the regulation of molting and metamorphosis in bed bugs with a goal to identify key players involved. qRT-PCR studies on the expression of genes known to be involved in molting and metamorphosis showed high levels of Krüppel homolog 1 [Kr-h1, a transcription factor that plays key roles in juvenile hormone (JH) action] mRNA in the penultimate nymphal stage (N4). However, low levels of Kr-h1 mRNA were detected in the fifth and last nymphal stage (N5). Knockdown of Kr-h1 in N4 resulted in a precocious development of adult structures. Kr-h1 maintains the immature stage by suppressing E93 (early ecdysone response gene) in N4. E93 expression increases during the N5 in the absence of Kr-h1 and promotes the development of adult structures. Knockdown of E93 in N5 results in the formation of supernumerary nymphs. The role of JH in the suppression of adult structures through interaction with Kr-h1 and E93 was also studied by the topical application of JH analog, methoprene, to N5. Methoprene induced Kr-h1 and suppressed E93 and induced formation of the supernumerary nymph. These data show interactions between Kr-h1, E93 and JH in the regulation of metamorphosis...Continue Reading

References

Sep 13, 2000·Molecular Cell·C Y LeeE H Baehrecke
Sep 10, 2004·Annual Review of Entomology·Xavier BellésMaria-Dolors Piulachs
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Deniz F ErezyilmazJames W Truman
Sep 14, 2006·Annual Review of Entomology·Klaus Reinhardt, Michael T Siva-Jothy
Apr 2, 2009·JAMA : the Journal of the American Medical Association·Jerome Goddard, Richard deShazo
Sep 2, 2009·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·M Jane Pritchard, Stephen W Hwang
Jan 5, 2011·Insect Biochemistry and Molecular Biology·Elisabeth MarchalJozef Vanden Broeck
Jul 14, 2011·Emerging Infectious Diseases·Christopher F Lowe, Marc G Romney
Dec 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jean-Philippe CharlesMarek Jindra
Dec 27, 2011·The American Journal of Medicine·Jerome Goddard, Richard de Shazo
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takumi KayukawaTetsuro Shinoda
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sang Woon ShinAlexander S Raikhel
Mar 16, 2013·Scientific Reports·Fang ZhuSubba R Palli
May 8, 2013·Bioscience, Biotechnology, and Biochemistry·Kenjiro FurutaTakahiro Shiotsuki
Jul 5, 2013·The American Journal of Case Reports·Stephanie BurrowsStephanie Susser
Mar 26, 2014·Developmental Biology·Vlastimil SmykalMarek Jindra
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enric UreñaDavid Martín
Jul 16, 2014·Insect Biochemistry and Molecular Biology·Jiasheng SongShutang Zhou
Nov 19, 2014·The American Journal of Tropical Medicine and Hygiene·Renzo SalazarMichael Z Levy

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Citations

Oct 21, 2016·Scientific Reports·Hemant Gujar, Subba Reddy Palli
Feb 14, 2018·Journal of Medical Entomology·Sanjay Basnet, Shripat T Kamble
Aug 1, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei GuoShutang Zhou
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yoshiyasu IshimaruTaro Mito
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marek Jindra
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Xavier Belles
May 13, 2020·Archives of Insect Biochemistry and Physiology·Duaa Musleh EidSubba Reddy Palli
Nov 15, 2019·Cellular and Molecular Life Sciences : CMLS·Jiasheng Song, Shutang Zhou
Oct 20, 2020·Current Opinion in Insect Science·David MartínXavier Franch-Marro
Feb 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xueli WangZhen Zou
Feb 27, 2022·Proceedings of the National Academy of Sciences of the United States of America·Genta OkudeRyo Futahashi

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Datasets Mentioned

BETA
SRX028107
SRX013985
3
SRX013984

Methods Mentioned

BETA
PCR

Software Mentioned

Per
Blast2go
DinoCapture2

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