Kruppel-like factor 1 (KLF1), KLF2, and Myc control a regulatory network essential for embryonic erythropoiesis.

Molecular and Cellular Biology
Christopher J PangMarie Trudel

Abstract

The Krüppel-like factor 1 (KLF1) and KLF2 positively regulate embryonic β-globin expression and have additional overlapping roles in embryonic (primitive) erythropoiesis. KLF1(-/-) KLF2(-/-) double knockout mice are anemic at embryonic day 10.5 (E10.5) and die by E11.5, in contrast to single knockouts. To investigate the combined roles of KLF1 and KLF2 in primitive erythropoiesis, expression profiling of E9.5 erythroid cells was performed. A limited number of genes had a significantly decreasing trend of expression in wild-type, KLF1(-/-), and KLF1(-/-) KLF2(-/-) mice. Among these, the gene for Myc (c-Myc) emerged as a central node in the most significant gene network. The expression of the Myc gene is synergistically regulated by KLF1 and KLF2, and both factors bind the Myc promoters. To characterize the role of Myc in primitive erythropoiesis, ablation was performed specifically in mouse embryonic proerythroblast cells. After E9.5, these embryos exhibit an arrest in the normal expansion of circulating red cells and develop anemia, analogous to KLF1(-/-) KLF2(-/-) embryos. In the absence of Myc, circulating erythroid cells do not show the normal increase in α- and β-like globin gene expression but, interestingly, have accelera...Continue Reading

References

Jul 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A De la ChapelleP A Marks
Jan 27, 1995·The Journal of Biological Chemistry·D DonzeJ J Bieker
Dec 1, 1993·Molecular and Cellular Biology·K J RiggsK Calame
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y FujiwaraS H Orkin
Sep 4, 1998·Science·T C HeK W Kinzler
Dec 22, 1998·Molecular and Cellular Biology·C V Dang
Dec 22, 1998·Transgenic Research·M A WaniJ B Lingrel
Aug 24, 1999·Nucleic Acids Research·S Philipsen, G Suske
Jul 10, 2001·The Journal of Biological Chemistry·J J Bieker
Oct 31, 2002·Blood·Kathleen E McGrathJames Palis
Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Apr 16, 2003·Genes & Development·Paula C FernandezBruno Amati
Jul 2, 2003·Molecular and Cellular Biology·Marcin RylskiMitchell J Weiss
Aug 2, 2003·Archives of Biochemistry and Biophysics·Stella Pelengaris, Mike Khan
Dec 10, 2003·Experimental Hematology·Margaret H Baron
Jan 20, 2004·Development·Richard MartinTrang Hoang
Apr 20, 2004·Blood·Achim C HeinrichUrsula Klingmüller
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Mar 9, 2005·Trends in Cell Biology·Mark J MurphyAndreas Trumpp
Jun 1, 2005·Molecular and Cellular Biology·Roy DrissenSjaak Philipsen
Oct 20, 2005·Blood·Nobuyuki MatsumotoScott L Friedman
Nov 3, 2005·Blood·Paul D KingsleyJames Palis
Dec 29, 2005·Blood·Denise HodgeAndrew Perkins
Feb 2, 2006·Molecular and Cellular Biology·Caroline ThiviergeMarie Trudel
Mar 2, 2006·Molecular and Cellular Biology·Kathryn A O'DonnellChi V Dang
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth

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Citations

Mar 12, 2013·Current Opinion in Hematology·Michael R Tallack, Andrew C Perkins
Jun 29, 2013·Hemoglobin·Ting WangDong-Zhi Li
May 8, 2016·Developmental Biology·Jerfiz D ConstanzoPier Paolo Scaglioni
Dec 3, 2016·PLoS Genetics·Laura FontenasMarcel Tawk
Jun 8, 2018·Prenatal Diagnosis·Zhou-Wei HuangMahesh Choolani
May 28, 2019·Journal of Cellular and Molecular Medicine·Anwei MaoGaofeng Pan
Dec 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lei XuDuonan Yu
Mar 8, 2020·Cellular Signalling·Almira Kurbegovic, Marie Trudel
Jul 22, 2021·Cancer Management and Research·Yuanchun LiWeihua Fu

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