Kruppel-like factors in an endothelial and vascular smooth muscle cell coculture model: impact of a diabetic environment and vitamin D

In Vitro Cellular & Developmental Biology. Animal
Tali Zitman-GalS Benchetrit

Abstract

Endothelial cells (EC) and vascular smooth muscle cells (VSMC) are involved in the development of local and diffuse vasculopathies by participating in inflammatory processes that can lead to uncontrolled vascular complications. Our aim was to study the possible interactions of EC and VSMC in an in vitro coculture model exposed to diabetic-like conditions and the effect of vitamin D on cellular pathways that might lead to an inflammatory response. EC and VSMC were isolated from different umbilical cords and stimulated in an in vitro coculture model in a diabetic-like environment and calcitriol for 24 h. Total RNA and protein were extracted from cells and analyzed for the expression of selected inflammatory-related markers. The EC-VSMC coculture in a diabetic-like environment induced the expression of inflammatory markers such as Kruppel-like factors, thioredoxin-interacting protein (TXNIP), IL-6, and IL-8. Addition of vitamin D to the EC-VSMC coculture induced selective changes in the inflammatory response. This model could lead to a better understanding of the interactions between EC and VSMC in the inflammatory processes involved in diabetes and emphasizes the role of vitamin D in the inflammatory response. The use of differen...Continue Reading

References

Nov 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J ThybergB A Bottger
Jun 1, 1997·The Journal of Clinical Investigation·S GuptaC Schindler
Jan 14, 1999·The New England Journal of Medicine·R Ross
Jul 10, 2001·The Journal of Biological Chemistry·J J Bieker
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiara BuonoAndrew H Lichtman
Sep 17, 2004·The Journal of Clinical Investigation·Kian Peng KohJordan S Pober
Feb 3, 2005·Current Opinion in Nephrology and Hypertension·Hideyuki Yamawaki, Bradford C Berk
Feb 19, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Jeng-Jiann ChiuShu Chien
Feb 19, 2005·Circulation Research·Zhiyong LinMukesh K Jain
Mar 29, 2006·Progress in Biophysics and Molecular Biology·Michael F Holick
Jun 23, 2007·Circulation Research·G Brandon Atkins, Mukesh K Jain
Dec 8, 2007·The Journal of Biological Chemistry·Jinghai WuJerry B Lingrel
Feb 16, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Amanda C DoranColeen A McNamara
Jul 9, 2009·The Journal of Clinical Endocrinology and Metabolism·Ozlem TarcinSema Akalin
Jan 22, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Tali Zitman-GalJacques Bernheim
May 25, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Charles S Wallace, George A Truskey
Jul 2, 2011·Journal of the American College of Cardiology·Ibhar Al MheidArshed A Quyyumi
Aug 6, 2013·Nutrients·Katharina KienreichStefan Pilz
Feb 12, 2014·Dermato-endocrinology·Uwe GröberMichael F Holick

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Jan 15, 2017·Cardiovascular Diabetology·Jason Kar Sheng LewRajesh Katare

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