KS-Detect - Validation of Solar Thermal PCR for the Diagnosis of Kaposi's Sarcoma Using Pseudo-Biopsy Samples

PloS One
Ryan SnodgrassDavid Erickson

Abstract

Resource-limited settings present unique engineering challenges for medical diagnostics. Diagnosis is often needed for those unable to reach central healthcare systems, making portability and independence from traditional energy infrastructure essential device parameters. In 2014, our group presented a microfluidic device that performed a solar-powered variant of the polymerase chain reaction, which we called solar thermal PCR. In this work, we expand on our previous effort by presenting an integrated, portable, solar thermal PCR system targeted towards the diagnosis of Kaposi's sarcoma. We call this system KS-Detect, and we now report the system's performance as a diagnostic tool using pseudo-biopsy samples made from varying concentrations of human lymphoma cell lines positive for the KS herpesvirus (KSHV). KS-Detect achieved 83% sensitivity and 70% specificity at high (≥ 10%) KSHV+ cell concentrations when diagnosing pseudo-biopsy samples by smartphone image. Using histology, we confirm that our prepared pseudo-biopsies contain similar KSHV+ cell concentrations as human biopsies positive for KS. Through our testing of samples derived from human cell lines, we validate KS-Detect as a viable, portable KS diagnostic tool, and we...Continue Reading

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Citations

Feb 6, 2017·Advanced Materials·Xinchun TianLudovico Cademartiri
Oct 8, 2016·Lab on a Chip·Christian D AhrbergBong Geun Chung
Nov 8, 2018·Lab on a Chip·Kamalalayam Rajan SreejithNam-Trung Nguyen
Mar 23, 2017·American Journal of Clinical Dermatology·Johann W Schneider, Dirk P Dittmer
Feb 2, 2019·Nature Reviews. Disease Primers·Ethel CesarmanDenise Whitby
Mar 25, 2019·Nature Biomedical Engineering·Ryan SnodgrassDavid Erickson

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Methods Mentioned

BETA
biopsy
PCR
chip
nucleic acid amplification
biopsies
electrophoresis
chips

Software Mentioned

GeneXpert
Detect
ImageJ
ImageJ Analyzer
- Detect
HALO image
HALO
KS

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