Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes.

The Journal of Biological Chemistry
Colin ConwayJ D Barry

Abstract

Trypanosome antigenic variation, involving differential expression of variant surface glycoprotein (VSG) genes, has a strong association with telomeres and with DNA recombination. All expressed VSGs are telomeric, and differential activation involves recombination into the telomeric environment or silencing/activation of subtelomeric promoters. A number of pathogen contingency gene systems associated with immune evasion involve telomeric loci, which has prompted speculation that chromosome ends provide conditions conducive for the operation of rapid gene switching mechanisms. Ku is a protein associated with eukaryotic telomeres that is directly involved in DNA recombination and in gene silencing. We have tested the hypothesis that Ku in trypanosomes is centrally involved in differential VSG expression. We show, via the generation of null mutants, that trypanosome Ku is closely involved in telomere length maintenance, more so for a transcriptionally active than an inactive telomere, but exhibits no detectable influence on DNA double strand break repair. The absence of Ku and the consequent great shortening of telomeres had no detectable influence either on the rate of VSG switching or on the silencing of the telomeric promoters ...Continue Reading

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Kitten, A G Barbour
Jan 1, 1994·Current Biology : CB·E R MoxonR E Lenski
Apr 5, 1996·The Journal of Biological Chemistry·G J MagesE L Winnacker
Apr 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G A Cross
Mar 1, 1997·Nature Genetics·M A Vega-PalasE Di Mauro
May 9, 1998·Molecular and Biochemical Parasitology·J E DonelsonN M El-Sayed
May 9, 1998·Molecular and Biochemical Parasitology·J D BarryB Wymer
May 25, 1999·Cell·J D GriffithT de Lange
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·F Pâques, J E Haber
Jun 23, 1999·Mutation Research·C Featherstone, S P Jackson
Aug 24, 1999·Molecular and Cellular Biology·N P RobinsonJ D Barry
Aug 24, 1999·Molecular and Cellular Biology·J A Downs, S P Jackson
Oct 26, 1999·Current Biology : CB·C Featherstone, S P Jackson
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·K G Murti, D M Prescott
Mar 1, 2000·Trends in Biochemical Sciences·L Aravind, E V Koonin
Apr 8, 2000·Trends in Biochemical Sciences·A Kass-Eisler, C W Greider
May 16, 2000·The EMBO Journal·D HornA K Ingram
Oct 12, 2000·Molecular Biology of the Cell·P Baumann, T R Cech
Oct 25, 2000·Molecular and Cellular Biology·N GrandinM Charbonneau
Jan 4, 2001·Nature Genetics·S E PetersonD E Gottschling
Feb 7, 2001·The EMBO Journal·J L Muñoz-JordánJ D Griffith
Feb 13, 2001·Infection and Immunity·J R Stringer, S P Keely
Mar 16, 2001·Current Opinion in Genetics & Development·D Shore
Mar 30, 2001·The Journal of Biological Chemistry·R L WoodardW S Dynan
May 10, 2001·Current Opinion in Cell Biology·K DubranaS M Gasser

❮ Previous
Next ❯

Citations

Sep 1, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·David Horn, J David Barry
May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mrinal Kanti BhattacharyyaNirbhay Kumar
Mar 24, 2004·Trends in Parasitology·Richard McCulloch
Jan 28, 2003·International Journal for Parasitology·J D BarryR McCulloch
May 14, 2003·Molecular and Biochemical Parasitology·Karen SheaderGloria Rudenko
Dec 13, 2006·Nature Reviews. Microbiology·Oliver DreesenGeorge A M Cross
Oct 2, 2002·Molecular Microbiology·Colin ConwayRichard McCulloch
Dec 17, 2004·Nucleic Acids Research·Christian J JanzenGeorge A M Cross
Dec 6, 2005·Nucleic Acids Research·Chris Proudfoot, Richard McCulloch
May 15, 2004·Molecular and Cellular Biology·Kyungjae MyungEric A Hendrickson
Jun 1, 2005·Molecular and Cellular Biology·Bibo LiGeorge A M Cross
Jun 11, 2004·Annual Review of Biochemistry·Agata Smogorzewska, Titia de Lange
Jul 11, 2006·Annual Review of Genetics·Karel RihaDorothy E Shippen
Jan 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Karel Riha, Dorothy E Shippen
Jan 11, 2007·The Journal of Cell Biology·David Landeira, Miguel Navarro
May 8, 2007·Trends in Microbiology·Miguel NavarroDavid Landeira
Aug 4, 2004·Current Opinion in Microbiology·Etienne PaysDavid Pérez-Morga
Nov 18, 2005·Molecular and Biochemical Parasitology·Chris Proudfoot, Richard McCulloch
Jun 28, 2005·DNA Repair·Timothy S Fisher, Virginia A Zakian
Aug 17, 2014·Biochemical and Biophysical Research Communications·Na ZhaoXichen Zhang
Sep 26, 2013·The Biochemical Journal·Ambuj K Kushwaha, Anne Grove
Aug 1, 2016·Molecular and Biochemical Parasitology·Samson O ObadoKirk W Deitsch
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Marie-Michelle GenoisJean-Yves Masson
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Andrew H LeeDavid A Fidock
Sep 9, 2010·Essays in Biochemistry·Gloria Rudenko
May 4, 2004·Nature Reviews. Molecular Cell Biology·Jessica A Downs, Stephen P Jackson
Aug 23, 2003·The Journal of Biological Chemistry·Joanna S Bell, Richard McCulloch
May 16, 2002·The Journal of Biological Chemistry·Nicholas P RobinsonJ David Barry
May 28, 2011·Molecular Microbiology·Rachel DobsonRichard McCulloch
Sep 25, 2017·Frontiers in Microbiology·Andrew A KelsoMichael G Sehorn
Dec 4, 2003·Molecular Microbiology·Joanna S BellRichard McCulloch
Jun 25, 2015·Microbiology Spectrum·Richard McCullochJames P J Hall

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