Kunitz type protease inhibitor EgKI-1 from the canine tapeworm Echinococcus granulosus as a promising therapeutic against breast cancer

PloS One
Shiwanthi L RanasingheDonald P McManus

Abstract

EgKI-1, a member of the Kunitz type protease inhibitor family, is highly expressed by the oncosphere of the canine tapeworm Echinococcus granulosus, the stage that is infectious to humans and ungulates, giving rise to a hydatid cyst localized to the liver and other organs. Larval protoscoleces, which develop within the hydatid cyst, have been shown to possess anti-cancer properties, although the precise molecules involved have not been identified. We show that recombinant EgKI-1 inhibits the growth and migration of a range of human cancers including breast, melanoma and cervical cancer cell lines in a dose-dependent manner in vitro without affecting normal cell growth. Furthermore, EgKI-1 treatment arrested the cancer cell growth by disrupting the cell cycle and induced apoptosis of cancer cells in vitro. An in vivo model of triple negative breast cancer (MDA-MB-231) in BALB/c nude mice showed significant tumor growth reduction in EgKI-1-treated mice compared with controls. These findings indicate that EgKI-1 shows promise for future development as an anti-cancer therapeutic.

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Citations

Nov 30, 2019·Parasite : Journal De La Société Française De Parasitologie·Sirous Mehrani MoghaddamEhsan Ahmadpour
Oct 15, 2019·Frontiers in Pharmacology·Wang GuanJinxin Zhang
Nov 9, 2019·Scientific Reports·Shiwanthi L RanasingheDonald P McManus
Dec 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hong YouDonald P McManus
Feb 6, 2020·Trends in Biochemical Sciences·Zachary R CrookJames M Olson
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karina Margareti Alencar de BarrosMaria Lígia Rodrigues Macedo

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Methods Mentioned

BETA
Hi-trap
flow cytometry

Software Mentioned

FlowJo
GraphPad Prism
ModFit LT
IncuCyte
Marker View
Analyst TF
ProteinPilot
ImageJ
PeakView
ImageScope

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