Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells

Journal of Clinical Medicine
Jung Sun MinSunoh Kwon

Abstract

Kurarinone is a prenylated flavonone isolated from the roots of Sophora flavescens. Among its known functions, kurarinone has both anti-apoptotic and anti-inflammatory properties. Coronaviruses (CoVs), including HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2, are the causative agents of respiratory virus infections that range in severity from the common cold to severe pneumonia. There are currently no effective treatments for coronavirus-associated diseases. In this report, we examined the anti-viral impact of kurarinone against infection with the human coronavirus, HCoV-OC43. We found that kurarinone inhibited HCoV-OC43 infection in human lung fibroblast MRC-5 cells in a dose-dependent manner with an IC50 of 3.458 ± 0.101 μM. Kurarinone inhibited the virus-induced cytopathic effect, as well as extracellular and intracellular viral RNA and viral protein expression. Time-of-addition experiments suggested that kurarinone acted at an early stage of virus infection. Finally, we found that HCoV-OC43 infection increased the autophagic flux in MRC-5 cells; kurarinone inhibited viral replication via its capacity to impair the virus-induced autophagic flux. As such, we suggest that kurarinone may be a useful therapeutic for the treatment...Continue Reading

References

Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zsolt TallóczyBeth Levine
Dec 31, 2003·The Journal of Biological Chemistry·Erik PrenticeMark R Denison
Oct 20, 2009·Cell Host & Microbe·Pierre-Emmanuel JoubertMathias Faure
May 18, 2010·Cellular & Molecular Immunology·Anna Hing-Yee LawAllan Sik-Yin Lau
Dec 3, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jianhui MaHongbing Zhang
Aug 31, 2012·Experimental & Molecular Medicine·Ok Won SeoYoung Myeong Kim
Dec 4, 2012·Viruses·Helena J Maier, Paul Britton
Oct 24, 2014·Journal of Virology·Sophie SagnierLucile Espert
Dec 3, 2014·Antiviral Research·Hai-yan ZhuMei-qing Feng
Feb 2, 2016·Current Opinion in Virology·Chantal Bem ReuskenBart L Haagmans
Apr 1, 2016·Scientific Reports·Longjun GuoYue Wang
Jul 12, 2017·The Journal of Experimental Medicine·Bin CaoIndira U Mysorekar
Dec 21, 2017·Viruses·Zhenlong LiuBenoit Barbeau
Mar 21, 2018·Nature Reviews. Microbiology·Younho ChoiJae U Jung
May 10, 2018·Scientific Reports·Katarzyna OwczarekKrzysztof Pyrc
Dec 5, 2018·Frontiers in Cell and Developmental Biology·Liyana AhmadVanessa Sancho-Shimizu
Jun 15, 2019·Cell·Lorenzo Galluzzi, Douglas R Green
Apr 1, 2020·International Journal of Biological Sciences·Naidi Yang, Han-Ming Shen
Apr 11, 2020·The New England Journal of Medicine·Jonathan GreinTimothy Flanigan

❮ Previous
Next ❯

Citations

Dec 17, 2020·Cells·Blanca Estela García-PérezNayeli Shantal Castrejón-Jiménez
Aug 28, 2021·Cells·Ankit Shroff, Taras Y Nazarko

❮ Previous
Next ❯

Methods Mentioned

BETA
reverse transcription PCR
Assay
fluorescence microscopy
Infection
light microscopy

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.