KV1 channels identified in rodent myelinated axons, linked to Cx29 in innermost myelin: support for electrically active myelin in mammalian saltatory conduction

Journal of Neurophysiology
J E RashJ I Nagy

Abstract

Saltatory conduction in mammalian myelinated axons was thought to be well understood before recent discoveries revealed unexpected subcellular distributions and molecular identities of the K(+)-conductance pathways that provide for rapid axonal repolarization. In this study, we visualize, identify, localize, quantify, and ultrastructurally characterize axonal KV1.1/KV1.2 channels in sciatic nerves of rodents. With the use of light microscopic immunocytochemistry and freeze-fracture replica immunogold labeling electron microscopy, KV1.1/KV1.2 channels are localized to three anatomically and compositionally distinct domains in the internodal axolemmas of large myelinated axons, where they form densely packed "rosettes" of 9-nm intramembrane particles. These axolemmal KV1.1/KV1.2 rosettes are precisely aligned with and ultrastructurally coupled to connexin29 (Cx29) channels, also in matching rosettes, in the surrounding juxtaparanodal myelin collars and along the inner mesaxon. As >98% of transmembrane proteins large enough to represent ion channels in these specialized domains, ∼500,000 KV1.1/KV1.2 channels define the paired juxtaparanodal regions as exclusive membrane domains for the voltage-gated K(+)conductance that underlies ...Continue Reading

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Citations

May 5, 2020·The Journal of Cell Biology·Katy L H Marshall-PhelpsDavid A Lyons
Nov 15, 2017·Histochemistry and Cell Biology·Carola Meier, Anja Beckmann
Mar 19, 2019·Frontiers in Cellular Neuroscience·Bruno A CisternaCarlos Puebla
Feb 15, 2019·Frontiers in Cellular Neuroscience·Sebastián VejarJuan A Orellana
Sep 15, 2020·Science Advances·Hyuk-Joon LeeJae-Sung Woo
Oct 18, 2019·Frontiers in Cellular Neuroscience·Pepe Alcami, Ahmed El Hady
Jan 1, 2021·Frontiers in Cell and Developmental Biology·Inês Lago-BaldaiaSarah D Ackerman
Dec 31, 2020·Life·Delphine Pinatel, Catherine Faivre-Sarrailh

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