PMID: 8592133Feb 1, 1996Paper

L-3,4-dihydroxyphenylalanine increases the quantal size of exocytotic dopamine release in vitro

Journal of Neurochemistry
E PothosD Sulzer

Abstract

The catecholamine precursor L-3,4-dihydroxyphenylalanine (L-DOPA) is used to augment striatal dopamine (DA), although its mechanism of altering neurotransmission is not well understood. We observed the effects of L-DOPA on catecholamine release in ventral midbrain neuron and PC12 pheochromocytoma cell line cultures. In ventral midbrain neuron cultures exposed to 40 mM potassium-containing media, L-DOPA (100 microM for 1 h) increased DA release by > 10-fold. The elevated extracellular DA levels were not significantly blocked by the DA/norepinephrine transport inhibitor nomifensine, demonstrating that reverse transport through catecholamine-uptake carriers plays little role in this release. In PC12 cells, where DA release from individual secretory vesicles can be observed, L-DOPA (50 microM for 1 h) elevated DA release in high-potassium media by 370%. Amperometric measurements demonstrated that L-DOPA (50 microM for 40-70 min) did not raise the frequency of vesicular exocytosis but increased the average size of quantal release to at least 250% of control levels. Together, these findings suggest that L-DOPA can increase stimulation-dependent transmitter release from DA cells by augmenting cytosolic neurotransmitter, leading to inc...Continue Reading

Citations

Mar 11, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Varvara E DyakonovaDmitri A Sakharov
Jun 1, 2020·Molecular Neurobiology·Nicolas PietrancostaJeffrey D Erickson
Aug 28, 2020·Brain : a Journal of Neurology·Madeleine E SharpDaphna Shohamy
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·D SulzerL Zecca
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