PMID: 7086829Jan 1, 1982Paper

L-[4-11C]aspartic acid: enzymatic synthesis, myocardial uptake, and metabolism

Journal of Medicinal Chemistry
J R BarrioF J Baumgartner

Abstract

Sterile, pyrogen-free L-[4-11C]aspartic acid was prepared from 11CO2 using phosphoenolpyruvate carboxylase and glutamic/oxaloacetic acid transaminase immobilized on Sepharose supports to determine if it is a useful indicator for in vivo, noninvasive determination of myocardial metabolism. An intracoronary bolus injection of L-[4-11C]aspartic acid into dog myocardium showed a triexponential clearance curve with maximal production of 11CO2 100 s after injection. Inactivation of myocardial transaminase activity modified the tracer clearance and inhibited the production of 11CO2. Positron-computed tomography imaging showed that the 11C activities retained in rhesus monkey myocardium are higher than those observed in dog heart after intravenous injection of L-[4-11C]aspartic acid. These findings demonstrated the rapid incorporation of the carbon skeleton of L-aspartic acid into the tricarboxylic acid cycle after enzymatic transamination in myocardium and suggested that L-[4-11C]aspartic acid could be of value for in vivo, noninvasive assessment of local myocardial metabolism.

Citations

Dec 1, 1985·Pflügers Archiv : European journal of physiology·O I PisarenkoI M Studneva
Jan 1, 1984·Neurosurgical Review·Y L YamamotoW H Feindel
Nov 29, 2013·Journal of Labelled Compounds & Radiopharmaceuticals·Johannes Ermert, H Coenen
Feb 8, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Aleksandra PekošakAlbert D Windhorst
Dec 13, 2017·Journal of Labelled Compounds & Radiopharmaceuticals·Eunice S da SilvaJordi Llop
Jan 1, 1986·European Journal of Nuclear Medicine·H R Schelbert

Related Concepts

SGOT - Glutamate oxaloacetate transaminase
Aspartic Acid, Magnesium-Potassium (2:1:2) Salt
Carbon Radioisotopes
Canis familiaris
Myocardium
Phosphoenolpyruvate Carboxylase

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