PMID: 7932648Jun 1, 1994Paper

L-alanine absorption in human intestinal Caco-2 cells driven by the proton electrochemical gradient

The Journal of Membrane Biology
D T ThwaitesN L Simmons

Abstract

In human Caco-2 intestinal epithelial layers, L-alanine absorption can be energized by a proton gradient across the brush-border membrane. Acidification of the apical medium, even in Na(+)-free media, is associated with a saturable net transepithelial absorption of L-alanine. L-Alanine transport causes cytosolic acidification consistent with proton/amino acid symport. L-Alanine transport in Na(+)-free media is rheogenic, stimulating an inward short-circuit current in voltage-clamped epithelial monolayers. By measurement of rapid L-alanine influx across the apical membrane, L-alanine-stimulated inward short-circuit current and intracellular acidification in the same cell batch, we estimate L-alanine/proton stoichiometry to be 1:0.62 +/- 0.25 (SD) (short-circuit current) or 1:0.73 +/- 0.19 (intracellular acidification). From competition studies, it is likely that L-proline, alpha-aminoisobutyric acid, and beta-alanine, but not L-valine and L-serine, are substrates for proton-linked, substrate transport in the brush border of Caco-2 cells.

Citations

May 3, 2003·Biochemical and Biophysical Research Communications·Zhong ChenDavid T Thwaites
Mar 11, 2000·British Journal of Pharmacology·D T ThwaitesN L Simmons
Jan 13, 2005·British Journal of Pharmacology·David J KennedyDavid T Thwaites
Mar 1, 1995·British Journal of Pharmacology·D T ThwaitesN L Simmons
Oct 23, 2014·Therapeutic Delivery·Martha Kampp NøhrCarsten Uhd Nielsen
Apr 20, 2011·British Journal of Pharmacology·David T Thwaites, Catriona M H Anderson
Nov 25, 2006·Biochimica Et Biophysica Acta·David T Thwaites, Catriona M H Anderson
Jan 31, 1997·Biochimica Et Biophysica Acta·K HashimotoM Shimizu
May 16, 2006·Amino Acids·L MetznerM Brandsch
Mar 10, 2018·Nutrients·Paulus G M JochemsPrescilla V Jeurink
May 1, 2007·Experimental Physiology·David T Thwaites, Catriona M H Anderson
Jan 16, 2008·Physiological Reviews·Stefan Bröer
Nov 14, 2013·American Journal of Physiology. Endocrinology and Metabolism·Anne JensenCarsten Uhd Nielsen
Sep 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin FoltzHannelore Daniel
Aug 24, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M DíazA Lorenzo
Apr 18, 2002·The Journal of Biological Chemistry·Michael BollHannelore Daniel
Dec 28, 2010·Physiology·Catriona M H Anderson, David T Thwaites
Jul 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Honglin FengDavid T Thwaites
Nov 13, 2020·Amino Acids·Ryosei SakaiTakahiro Narita
Mar 8, 1995·Biochimica Et Biophysica Acta·D T ThwaitesN L Simmons
Feb 13, 2001·Gastroenterology·G R ChristieB H Hirst

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.