Feb 28, 2013

L-Arginine enhances cell proliferation and reduces apoptosis in human endometrial RL95-2 cells

Reproductive Biology and Endocrinology : RB&E
Jonathan M GreenePeter L Ryan


L-arginine is considered to be one of the most versatile amino acids due to the fact that it serves as a precursor for many important molecules in cellular physiology. When supplemented in the diet, L-arginine can increase the number of implantation sites in mice and rats, suggesting an effect at the level of the endometrium. To this end, this study determined the effect that L-arginine has on apoptosis and cell proliferation in human endometrial RL95-2 cells. L-arginine at physiological (200 micromol/L) and supra-physiological (800 micromol/L) concentrations increased cell proliferation at days 2 and 4 post-treatment with a dose-dependent effect being observed on day 2. Additionally, inhibition of nitric oxide (NO) synthase and arginase, which are responsible for the conversion of L-arginine to NO and polyamines, respectively, reduced the proliferative effect of L-arginine. L-arginine also decreased the proportion of cells with TUNEL positive nuclei and increased the ratio of cells with healthy mitochondria compared to cells with a disrupted mitochondrial membrane potential, indicating that L-arginine prevents mitochondrial mediated apoptosis in endometrial RL95-2 cells. Furthermore, exposure to L-arginine did not affect total...Continue Reading

Mentioned in this Paper

Arginine hydrochloride
Polyamine Biosynthetic Process
Apoptosis, Intrinsic Pathway
Amino Acids, I.V. solution additive
Protein Phosphorylation
Blastocyst Implantation, Natural
Cell Proliferation
Carcinoma in Situ of Endometrium

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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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