Abstract
Protease-resistant, misfolded isoforms (PrP(Sc)) of a normal cellular prion protein (PrP(C)) in the bodily fluids, including blood, urine, and saliva, are expected to be useful diagnostic markers of prion diseases, and nonhuman primate models are suited for performing valid diagnostic tests for human Creutzfeldt-Jakob disease (CJD). We developed an effective amplification method for PrP(Sc) derived from macaques infected with the atypical L-type bovine spongiform encephalopathy (L-BSE) prion by using mouse brain homogenate as a substrate in the presence of polyanions and L-arginine ethylester. This method was highly sensitive and detected PrP(Sc) in infected brain homogenate diluted up to 10(10) by sequential amplification. This method in combination with PrP(Sc) precipitation by sodium phosphotungstic acid is capable of amplifying very small amounts of PrP(Sc) contained in the cerebrospinal fluid (CSF), saliva, urine, and plasma of macaques that have been intracerebrally inoculated with the L-BSE prion. Furthermore, PrP(Sc) was detectable in the saliva or urine samples as well as CSF samples obtained at the preclinical phases of the disease. Thus, our novel method may be useful for furthering the understanding of bodily fluid ...Continue Reading
References
Feb 1, 1991·Bio/technology·J Buchner, R Rudolph
Jun 14, 1991·Science·S B Prusiner
Aug 6, 1991·Biochemistry·B W CaugheyW S Caughey
Oct 31, 1987·The Veterinary Record·G A WellsR Bradley
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M PanF E Cohen
Apr 6, 1996·Lancet·R G WillP G Smith
Oct 23, 1997·Nature·A F HillP Lantos
Oct 15, 1998·Nature Medicine·J SafarS B Prusiner
Apr 3, 2001·Annual Review of Neuroscience·J Collinge
Jul 22, 2004·European Journal of Biochemistry·Kentaro ShirakiMasahiro Takagi
Oct 2, 2004·Biotechnology Progress·Kouhei TsumotoTsutomu Arakawa
May 27, 2005·The Journal of Biological Chemistry·Nathan R DeleaultSurachai Supattapone
Sep 18, 2007·The Journal of General Virology·Yuichi MurayamaShirou Mohri
Feb 1, 2008·Journal of Virology·Qingzhong KongPierluigi Gambetti
Aug 21, 2008·PloS One·Emmanuel E ComoyJean-Philippe Deslys
Dec 3, 2008·Emerging Infectious Diseases·Vincent BéringueHubert Laude
Jul 16, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·J W Ironside
Aug 11, 2010·Archives of Neurology·Ana LukicSimon Mead
Oct 20, 2010·PloS One·Yuichi MurayamaShirou Mohri
Jan 29, 2011·International Journal of Molecular Medicine·Akikazu SakudoYasuharu Tanaka
May 14, 2011·Neuroscience Letters·Takashi YokoyamaMasanori Morita
Jan 21, 2012·Emerging Infectious Diseases·Nadine Mestre-FrancésJean-Michel Verdier
Apr 25, 2012·Methods in Molecular Biology·Marcelo A BarriaClaudio Soto
Jun 20, 2014·PLoS Pathogens·Caroline LacrouxOlivier Andréoletti
Jul 16, 2014·The Journal of General Virology·Yuichi MurayamaTetsutaro Sata
Aug 8, 2014·The New England Journal of Medicine·Fabio ModaClaudio Soto
Dec 3, 2014·Virus Research·Paula Saá, Larisa Cervenakova
Sep 9, 2015·Transfusion·Masatoshi OshitaMasanori Morita
Citations
Dec 18, 2019·The Journal of General Virology·Erin E McNultyCandace K Mathiason
Jun 27, 2020·The Journal of General Virology·Anne Balkema-BuschmannMartin H Groschup
Nov 9, 2016·The Journal of Biological Chemistry·Morikazu ImamuraYuichi Murayama
Mar 25, 2020·Biomolecules·Hasier ErañaJoaquín Castilla
Mar 11, 2017·Prion·Matthew J O'ConnorKevin C Gough
Sep 2, 2021·Transboundary and Emerging Diseases·Yong-Chan KimByung-Hoon Jeong