L-Ascorbic acid accumulation in fruit of Ribes nigrum occurs by in situ biosynthesis via the L-galactose pathway

Functional Plant Biology : FPB
Robert D HancockRoberto Viola

Abstract

Blackcurrant (Ribes nigrum L.) is a widely grown commercial crop valued for its high vitamin C (l-ascorbic acid, AsA) content. In the present study, a systematic analysis of the mechanism of fruit AsA accumulation was undertaken. AsA accumulation occurred during fruit expansion and was associated with high in situ biosynthetic capacity via the l-galactose pathway and low rates of turnover. Cessation of AsA accumulation was associated with reduced biosynthesis and increased turnover. Translocation of AsA from photosynthetic or vegetative tissues contributed little to fruit AsA accumulation. Manipulation of substrate availability by defoliation had no effect on fruit AsA concentration but significantly reduced fruit yields. Supply of the AsA precursor l-galactono-1,4-lactone to intact, attached fruit transiently increased fruit AsA concentration which rapidly returned to control levels after removal of the compound. These data suggest strong developmental, metabolic and genetic control of AsA accumulation in blackcurrant fruit and indicate the potential for breeding high AsA cultivars.

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Citations

Oct 31, 2012·Journal of Experimental Botany·Noé GestRebecca Stevens
Feb 20, 2013·Annals of Botany·Christopher J AtkinsonHelen Longbottom
Jun 10, 2011·Journal of the Science of Food and Agriculture·Vanessa J MelinoChristopher M Ford
Dec 1, 2014·Botanical Studies·Jelli Venkatesh, Se Won Park
Nov 9, 2019·Antioxidants & Redox Signaling·Christine H FoyerRobert D Hancock
Feb 8, 2020·Metabolomics : Official Journal of the Metabolomic Society·Ilka N AbreuChristine A Hackett
Oct 24, 2018·The Biochemical Journal·Rebecca A Dewhirst, Stephen C Fry

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