PMID: 7334369Dec 1, 1981Paper

L-Aspartate transport into plasma membrane vesicles derived from rat brain synaptosomes

Journal of Neurochemistry
J G MarvizónF Valdivieso

Abstract

Aspartate uptake by membrane vesicles derived from rat brain was investigated. The uptake is dependent on a Na+ gradient ([Na+] outside greater than [Na+] inside). Active transport of aspartate is strictly dependent upon the presence of sodium and maximal extent of transport is reached when both Na+ and Cl- ions are present. The uptake is transport into an osmotically active space and not a binding artifact as indicated by the effect of increasing the medium osmolarity. The uptake of aspartate is stimulated by a membrane potential (negative inside), as demonstrated by the effect of the ionophore carbonyl cyanide m-chlorophenylhydrazone and anions with different permeabilities. The presence of ouabain, an inhibitor of (Na+ + K+)-ATPase, does not affect aspartate transport. The kinetic analysis shows that aspartate is accumulated by two systems with different affinities, showing Km and Vmax values of similar order to those found in slightly "cruder" preparations. Inhibition of the L-aspartate uptake by D-aspartate and D- and L-glutamate indicates that a common carrier is involved in the process, this being stereospecific for the D- and L-glutamate stereoisomers.

References

Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D LichtshteinA J Blume
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Citations

Jun 1, 1997·Molecular Neurobiology·F ZafraC Giménez
May 6, 1982·Brain Research·M RecasensJ Benavides
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·U Albus, E Habermann
Dec 30, 1983·Biochimica Et Biophysica Acta·B I Kanner
Jan 1, 1986·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E HerreroC Gimenez
Nov 13, 2003·European Journal of Pharmacology·Carmen Aragón, Beatriz López-Corcuera
May 23, 2001·Progress in Neurobiology·N C Danbolt
May 1, 1989·European Journal of Biochemistry·B Lopez-Corcuera, C Aragon
May 1, 1983·Acta Physiologica Scandinavica·E R Korpi
Sep 1, 1983·Journal of Neurochemistry·V Adam-Vizi, R M Marchbanks
Feb 1, 1983·Journal of Neurochemistry·E HerreroF Valdivieso
Aug 1, 1986·Journal of Neurochemistry·F MayorF Mayor
Jun 1, 1986·Journal of Neurochemistry·J D Rothstein, B Tabakoff
Feb 27, 1986·Biochimica Et Biophysica Acta·M C Aragón, C Giménez
Jan 1, 1986·Journal of Neuroscience Research·J Ferkany, J T Coyle

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