L-Carnitine Reduces Oxidative Stress and Promotes Cells Differentiation and Bone Matrix Proteins Expression in Human Osteoblast-Like Cells

BioMed Research International
Ileana TerruzziAlessandro Rubinacci

Abstract

Bone fragility and associated fracture risk are major problems in aging. Oxidative stress and mitochondrial dysfunction play a key role in the development of bone fragility. Mitochondrial dysfunction is closely associated with excessive production of reactive oxygen species (ROS). L-Carnitine (L-C), a fundamental cofactor in lipid metabolism, has an important antioxidant property. Several studies have shown how L-C enhances osteoblastic proliferation and activity. In the current study, we investigated the potential effects of L-C on mitochondrial activity, ROS production, and gene expression involved in osteoblastic differentiation using osteoblast-like cells (hOBs) derived from elderly patients. The effect of 5mM L-C treatment on mitochondrial activity and L-C antioxidant activity was studied by ROS production evaluation and cell-based antioxidant activity assay. The possible effects of L-C on hOBs differentiation were assessed by analyzing gene and protein expression by Real Time PCR and western blotting, respectively. L-C enhanced mitochondrial activity and improved antioxidant defense of hOBs. Furthermore, L-C increased the phosphorylation of Ca2+/calmodulin-dependent protein kinase II. Additionally, L-C induced the phospho...Continue Reading

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Citations

Nov 11, 2019·Journal of Endocrinological Investigation·A MontesanoI Terruzzi
May 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alessandra DurazzoAntonello Santini
Dec 18, 2020·Animals : an Open Access Journal From MDPI·Janghan Choi, Woo Kyun Kim
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Apr 1, 2021·Life Sciences·Sulagna Mukherjee, Jong Won Yun
Aug 20, 2021·Metabolomics : Official Journal of the Metabolomic Society·Albe SwanepoelIlse du Preez

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Methods Mentioned

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PCR
Protein Assay
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Software Mentioned

Elements D
Nis
Prism
SPSS
Image J
GraphPad
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