L-cysteine stimulates hydrogen sulfide synthesis in myocardium associated with attenuation of ischemia-reperfusion injury

Journal of Cardiovascular Pharmacology and Therapeutics
David J ElseyGary F Baxter

Abstract

Hydrogen sulfide (H( 2)S) is a biological mediator produced by enzyme-regulated pathways from L-cysteine, which is a substrate for cystathionine-gamma-lyase (CSE). In myocardium, endogenously and exogenously administered H(2)S has been shown to protect against ischemia-reperfusion injury. We hypothesized that L-cysteine exerts its protective action through stimulation of H(2)S production. Rat isolated hearts were Langendorff-perfused and underwent 35-minute regional ischemia and 120-minute reperfusion. L-cysteine perfusion from 10 minutes before ischemia until 10 minutes after reperfusion limited infarct size in a concentration-dependent manner, maximal at 1 mmol/L (control 36.4% +/- 2.4% vs L-cysteine 24.3% +/- 3.4%, P < .05). This protective action was attenuated by the CSE inhibitor, DL-propargylglycine (PAG) 1 mmol/L (31.4 +/- 5.9%, not significant vs control) but administration of the CSE cofactor pyridoxal-5'-phosphate (PLP) 50 mumol/L did not enhance the effect of L-cysteine. Ten minutes normoxic perfusion with L-cysteine 1 mmol/L caused a 3-fold increase in myocardial H(2)S concentration (0.64 +/- 0.16 vs 2.01 +/- 0.07 mumol/g protein, P < .01), an effect that was significantly attenuated by PAG (1.17 +/- 0.15 mumol/g p...Continue Reading

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Citations

Feb 9, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M Scott Vandiver, Solomon H Snyder
Feb 19, 2016·British Journal of Pharmacology·Vincenzo BrancaleoneGiuseppe Cirino
Jun 13, 2015·Oxidative Medicine and Cellular Longevity·Dongdong WuYanzhang Li
Jun 19, 2014·Pflügers Archiv : European journal of physiology·Yusuke KumaiSteve F Perry
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Nov 26, 2011·Expert Review of Clinical Pharmacology·Xianfeng Gu, Yi Zhun Zhu
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Jul 23, 2021·Klinicheskaia laboratornaia diagnostika·Irina Gennadievna PopovaYu N Veselkova

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