L-cysteine supplementation upregulates glutathione (GSH) and vitamin D binding protein (VDBP) in hepatocytes cultured in high glucose and in vivo in liver, and increases blood levels of GSH, VDBP, and 25-hydroxy-vitamin D in Zucker diabetic fatty rats

Molecular Nutrition & Food Research
S K JainD Micinski

Abstract

Vitamin D binding protein (VDBP) status has an effect on and can potentially improve the status of 25(OH) vitamin D and increase the metabolic actions of 25(OH) vitamin D under physiological and pathological conditions. Diabetes is associated with lower levels of glutathione (GSH) and 25(OH) vitamin D. This study examined the hypothesis that upregulation of GSH will also upregulate blood levels of VDBP and 25(OH) vitamin D in type 2 diabetic rats. L-cysteine (LC) supplementation was used to upregulate GSH status in a FL83B hepatocyte cell culture model and in vivo using Zucker diabetic fatty (ZDF) rats. Results show that LC supplementation upregulates both protein and mRNA expression of VDBP and vitamin D receptor (VDR) and GSH status in hepatocytes exposed to high glucose, and that GSH deficiency, induced by glutamate cysteine ligase knockdown, resulted in the downregulation of GSH, VDBP, and VDR and an increase in oxidative stress levels in hepatocytes. In vivo, LC supplementation increased GSH and protein and mRNA expression of VDBP and vitamin D 25-hydroxylase (CYP2R1) in the liver, and simultaneously resulted in elevated blood levels of LC and GSH, as well as increases in VDBP and 25(OH) vitamin D levels, and decreased inf...Continue Reading

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Citations

Mar 9, 2019·The Kaohsiung Journal of Medical Sciences·Jun PengJian-Guang Xu
Jan 1, 2020·American Journal of Physiology. Endocrinology and Metabolism·Dongxia WangLiping Hao
Sep 2, 2020·Journal of the American College of Nutrition·Navya VyasSonal Sekhar Miraj
Jul 13, 2021·Gene Reports·Falaque Ul AfshanBashir Ahmad Ganai
Aug 3, 2021·International Journal of General Medicine·Rbab TahaAltaf Abdulkhaliq
Aug 6, 2021·Nutrition·Danilo Társio Mota BritoRoberto de Barros Silva

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