l-Galactono-gamma-lactone dehydrogenase from Arabidopsis thaliana, a flavoprotein involved in vitamin C biosynthesis

The FEBS Journal
Nicole G H LeferinkWillem J H van Berkel

Abstract

l-Galactono-1,4-lactone dehydrogenase (GALDH; ferricytochrome c oxidoreductase; EC 1.3.2.3) is a mitochondrial flavoenzyme that catalyzes the final step in the biosynthesis of vitamin C (l-ascorbic acid) in plants. In the present study, we report on the biochemical properties of recombinant Arabidopsis thaliana GALDH (AtGALDH). AtGALDH oxidizes, in addition to l-galactono-1,4-lactone (K(m) = 0.17 mm, k(cat) = 134 s(-1)), l-gulono-1,4-lactone (K(m) = 13.1 mm, k(cat) = 4.0 s(-1)) using cytochrome c as an electron acceptor. Aerobic reduction of AtGALDH with the lactone substrate generates the flavin hydroquinone. The two-electron reduced enzyme reacts poorly with molecular oxygen (k(ox) = 6 x 10(2) m(-1).s(-1)). Unlike most flavoprotein dehydrogenases, AtGALDH forms a flavin N5 sulfite adduct. Anaerobic photoreduction involves the transient stabilization of the anionic flavin semiquinone. Most aldonolactone oxidoreductases contain a histidyl-FAD as a covalently bound prosthetic group. AtGALDH lacks the histidine involved in covalent FAD binding, but contains a leucine instead (Leu56). Leu56 replacements did not result in covalent flavinylation but revealed the importance of Leu56 for both FAD-binding and catalysis. The Leu56 varia...Continue Reading

References

Aug 1, 1978·European Journal of Biochemistry·F Lederer
Nov 1, 1994·European Journal of Biochemistry·W K HuhS O Kang
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Mar 26, 1998·Protein Science : a Publication of the Protein Society·M MewiesN S Scrutton
Jun 30, 1998·Trends in Biochemical Sciences·M W FraaijeA Mattevi
Sep 24, 1999·Methods in Molecular Biology·P Macheroux
Oct 12, 1999·Plant Physiology·M W DaveyM C Van Montagu
Dec 10, 1999·The Journal of Biological Chemistry·M W FraaijeA Mattevi
Mar 1, 2000·Trends in Biochemical Sciences·M W Fraaije, A Mattevi
Sep 14, 2000·The Journal of Biological Chemistry·M W FraaijeA Mattevi
Sep 27, 2000·Critical Reviews in Biochemistry and Molecular Biology·N Smirnoff, G L Wheeler
Jan 14, 2003·Nature Biotechnology·Fernanda AgiusVictoriano Valpuesta
Jan 16, 2003·Biochimica Et Biophysica Acta·Marco H HeftiWillem J H van Berkel
Sep 5, 2003·The Journal of Biological Chemistry·Beata A Wolucka, Marc Van Montagu
Mar 1, 1958·The Biochemical Journal·L W MAPSON, E BRESLOW
Nov 27, 2004·Trends in Plant Science·Victoriano Valpuesta, Miguel A Botella
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shane R WilkinsonJohn M Kelly
Jan 16, 2007·The FEBS Journal·Carole L Linster, Emile Van Schaftingen
Oct 25, 2007·Biochemistry·A Kader Ould BoubacarFlorence Lederer

❮ Previous
Next ❯

Citations

Dec 25, 2012·Antioxidants & Redox Signaling·András SzarkaHan Asard
Apr 17, 2009·Plant Physiology·Nicole G H LeferinkWillem J H van Berkel
Nov 17, 2011·European Biophysics Journal : EBJ·Irene Díaz-MorenoMiguel A De la Rosa
Dec 24, 2015·Plant Physiology and Biochemistry : PPB·Siddique I Aboobucker, Argelia Lorence
Feb 27, 2013·The FEBS Journal·Manuel HervásWillem J H van Berkel
Apr 14, 2015·IUBMB Life·Biyun ChingYuen K Ip
Jan 9, 2015·Microbial Cell Factories·Joosu KuivanenPeter Richard
Mar 15, 2011·Biochimica Et Biophysica Acta·Elena V KudryashovaWillem J H van Berkel
May 9, 2014·Frontiers in Plant Science·Peter Schertl, Hans-Peter Braun
Jan 24, 2016·Biochimica Et Biophysica Acta·Nitya SubrahmanianPatrice Paul Hamel
Aug 6, 2014·Biochimica Et Biophysica Acta·Blas Moreno-BeltránIrene Díaz-Moreno
Sep 19, 2008·The Journal of Biological Chemistry·Bernard PineauRosine De Paepe
Jan 9, 2019·Frontiers in Plant Science·Simon StrobbeDominique Van Der Straeten
Dec 18, 2008·The Journal of Biological Chemistry·Nicole G H LeferinkWillem J H van Berkel
Nov 2, 2019·Antioxidants·Costantino PaciollaMaria Concetta de Pinto
Jan 13, 2021·Nature Chemical Biology·Mark DulchavskyFrederick Stull
Feb 19, 2008·Archives of Biochemistry and Biophysics·Nicole G H LeferinkWillem J H van Berkel
Oct 1, 2008·Trends in Plant Science·Carole L Linster, Steven G Clarke
Jun 29, 2010·Journal of Plant Physiology·Min OuyangLixin Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.