L-glutamate activates RhoA GTPase leading to suppression of astrocyte stellation

The European Journal of Neuroscience
Chun-Jung ChenAn-Na Chiang

Abstract

The actin cytoskeleton is known to support cellular morphological changes. Rho family small GTPases function as switching molecules to promote the convergence of both extracellular and intracellular signals in regulating cytoskeletal organization. Evidence indicates that L-glutamate suppresses morphological changes of astrocytes over a broad spectrum. To test the possibility that L-glutamate affects cytoskeletal reorganization, we investigated its effect on morphological changes induced by manganese exposure. L-glutamate concentration-dependently prevented and reversed manganese-induced astrocyte stellation and cytoskeletal disruption. The suppressive effect of L-glutamate on manganese-induced stellation was mediated by the activation of the glutamate transporter rather than ionotropic or metabotropic glutamate receptors. Pharmacological and biochemical approaches revealed the involvement of Ras homolog gene family, member A (RhoA) activation in L-glutamate-mediated suppression of manganese-induced stellation. The activation of RhoA by L-glutamate was partly through the up-regulation of guanine nucleotide exchange factor phosphorylation and was abrogated by competitive nonsubstrate inhibitors. Furthermore, the hyperphosphorylat...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T StorckW Stoffel
Apr 1, 1992·The Journal of Cell Biology·D M BaortoM L Shelanski
May 1, 1991·Neurochemical Research·M LedigF Wedler
Apr 1, 1990·Neurology·T I Mandybur, C C Chuirazzi
Jan 1, 1991·Annual Review of Cell Biology·A Bretscher
Dec 1, 1990·Journal of Neurochemistry·Y Cho, S Bannai
Feb 1, 1986·Annals of Neurology·S M Rothman, J W Olney
Jul 1, 1987·Physiological Reviews·J R Prohaska
Jan 1, 1981·International Review of Neurobiology·A Schousboe
Jul 2, 1984·Life Sciences·R SchwarczC Köhler
Oct 1, 1994·Journal of Neurophysiology·A AmatoD Attwell
Jan 1, 1993·Annual Review of Cell Biology·J Condeelis
Oct 17, 1996·Nature·N Zerangue, M P Kavanaugh
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·J L ArrizaS G Amara
Nov 14, 1997·Neurochemical Research·A S Hazell, M D Norenberg
Jan 7, 1998·Trends in Neurosciences·J L RidetF H Gage
Feb 7, 1998·Science·A Hall
Mar 14, 1998·Molecular Pharmacology·K ShimamotoT Nakajima
May 20, 1998·Journal of Neuroscience Research·A S BenderM D Norenberg
Dec 16, 1998·Experimental Cell Research·G J Ramakers, W H Moolenaar
Nov 18, 2000·Experimental Cell Research·A A SchmitzL Van Aelst
Nov 21, 2000·Current Opinion in Neurobiology·M G Todman, P J Benson
Nov 21, 2000·Current Opinion in Neurobiology·B A Barres, Y Barde
Feb 22, 2001·Annual Review of Physiology·A AraqueP G Haydon
Apr 18, 2001·Biological & Pharmaceutical Bulletin·K Abe, H Saito
Dec 14, 2001·Neuroreport·S L Liao, C J Chen
Jan 26, 2002·Microscopy Research and Technique·Seiji Miyata, Glenn I Hatton
May 16, 2002·Experimental Neurology·Chun-Jung Chen, Su-Lan Liao
Aug 23, 2002·Trends in Cell Biology·Bernhard Wehrle-Haller, Beat Imhof
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
May 24, 2003·Brain Research. Developmental Brain Research·Kazuho Abe, Miwa Misawa

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Citations

Sep 13, 2014·Oxidative Medicine and Cellular Longevity·Mauricio Olguín-AlbuerneJulio Morán
Mar 1, 2011·Neurochemistry International·Chun-Jung ChenChen-Jung Chang
Aug 31, 2010·Toxicology Letters·Cheng-Yi ChangChun-Jung Chen
Jun 1, 2015·Brain Structure & Function·Grégory GhézaliNathalie Rouach
May 18, 2011·Toxicology Letters·Su-Lan LiaoChun-Jung Chen
Sep 26, 2007·Journal of Neuroscience Research·Claudia LehmannJürgen Engele
Jul 16, 2008·Physiological Reviews·Dionysia T TheodosisStéphane H R Oliet
Jan 11, 2019·Neurochemical Research·Edna Suárez-PozosBabette Fuss

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