L-Glutamate Enhances Barrier and Antioxidative Functions in Intestinal Porcine Epithelial Cells

The Journal of Nutrition
Ning JiaoGuoyao Wu

Abstract

L-Glutamate (Glu) is a major amino acid in milk and postweaning diets for mammals (including pigs and human infants). However, effects of Glu on intestinal mucosal barrier and antioxidative functions are unknown. This study tested the hypothesis that Glu may enhance the barrier function of intestinal porcine epithelial cell line 1 (IPEC-1) cells by upregulating the expression of tight junction proteins. IPEC-1 cells were cultured with or without Glu in the presence or absence of 1 mmol/L diquat (an oxidant) for indicated time points. Cell numbers, transepithelial electrical resistance (TEER), mRNA, and protein abundance of glutamate transporter, the release of lactate dehydrogenase (LDH), and the abundance of tight junction proteins were determined. Compared with 0 mmol/L Glu, 0.5-, 1-, and 2 mmol/L Glu stimulated (P < 0.05) cell growth by 13-37% at 24 h and 12-34% at 48 h, respectively. In addition, 0.5 mmol/L Glu increased (P < 0.05) TEER (by 58% at 24 h and by 98% at 48 h, respectively). These effects of Glu were associated with increased mRNA abundance of Glu transporter solute carrier family 1 member 1 (SLC1A1) by 30-130% and protein abundance of excitatory amino acid transporter 3 (encoded by SLC1A1) by 19-34%, respective...Continue Reading

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Citations

Aug 24, 2017·Nutrients·Yulan LiuChien-An Andy Hu
Jan 24, 2019·Journal of Animal Physiology and Animal Nutrition·Xian TanYulong Yin
Feb 29, 2020·Journal of Inflammation Research·Junyan WangShuancheng Bai
Apr 15, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ning JiaoJingdong Yin
Apr 24, 2018·BioMed Research International·Fang HeYuanyi Peng
Aug 18, 2018·Amino Acids·Yongqing Hou, Guoyao Wu
Oct 17, 2020·Clinical Oral Investigations·Prem K SreenivasanYogitha Setty
Aug 24, 2021·Journal of Animal Physiology and Animal Nutrition·Santi Devi UpadhayaIn Ho Kim
Aug 31, 2021·Journal of Materials Science. Materials in Medicine·Mengchang XuSongwen Tan
Aug 31, 2021·Frontiers in Veterinary Science·Jiaxin ChenDayou Shi

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