L-Glutamine as a nitrogen source for Corynebacterium glutamicum: derepression of the AmtR regulon and implications for nitrogen sensing

Microbiology
Nadine RehmMichael Bott

Abstract

Corynebacterium glutamicum, a Gram-positive soil bacterium employed in the industrial production of various amino acids, is able to use a number of different nitrogen sources, such as ammonium, urea or creatinine. This study shows that l-glutamine serves as an excellent nitrogen source for C. glutamicum and allows similar growth rates in glucose minimal medium to those in ammonium. A transcriptome comparison revealed that the nitrogen starvation response was elicited when glutamine served as the sole nitrogen source, meaning that the target genes of the global nitrogen regulator AmtR were derepressed. Subsequent growth experiments with a variety of mutants defective in nitrogen metabolism showed that glutamate synthase is crucial for glutamine utilization, while a putative glutaminase is dispensable under the experimental conditions used. The gltBD operon encoding the glutamate synthase is a member of the AmtR regulon. The observation that the nitrogen starvation response was elicited at high intracellular l-glutamine levels has implications for nitrogen sensing. In contrast with other Gram-positive and Gram-negative bacteria such as Bacillus subtilis, Salmonella enterica serovar Typhimurium and Klebsiella pneumoniae, a drop in...Continue Reading

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Citations

Jun 29, 2013·Microbial Cell Factories·Jaide Vold Korgaard Jensen, Volker Fritz Wendisch
Aug 7, 2012·FEMS Microbiology Reviews·Luciano F HuergoMike Merrick
Oct 19, 2016·FEMS Microbiology Letters·Julia BuergerAndreas Burkovski
Aug 28, 2021·International Journal of Molecular Sciences·Ning LiuJian-Zhong Xu

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